检测支气管肺泡灌洗液中脆性组氨酸三联体与β-微管蛋白在肺癌诊断中的价值  

Value of detection of FHIT andβ-tubulin in bronchoalveolar lavage fluid in dignosis of lung cancer

作　　者：陈宇平 穆传勇[2] 宋丽丽 王一江 王宁江 邹雯 周林福[3] 

机构地区：[1]江苏盛泽医院呼吸病科,215228 [2]苏州大学附属第一医院呼吸病科 [3]南京医科大学第一附属医院呼吸病科

出　　处：《江苏医药》2015年第15期1762-1763,1767,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(81401943;81370133;81170018);江苏省医学重点人才项目(RC2011066);江苏省333高层次人才培养工程(201115);苏州市科技计划项目(SYS201462)

摘　　要：目的探讨支气管肺泡灌洗液(BALF)中脆性组氨酸三联体(FHIT)和β-微管蛋白(β-tubulin)的表达及其在肺癌诊断中的价值。方法确诊肺癌患者53例及肺部良性疾病患者22例,应用ELISA法检测病灶所在段BALF中FHIT和β-tubulin表达。结果与肺部良性疾病患者比较,肺癌患者BALF中FHIT含量较低,而β-tubulin含量较高(P<0.05)。肺癌患者中,鳞癌和小细胞肺癌患者BALF中的FHIT含量较腺癌患者低,而β-tubulin含量较高(P<0.05)。不同分期肺癌患者的FHIT和β-tubulin含量差异无统计学意义(P>0.05)。结论肺癌患者BALF中FHIT含量降低、β-tubulin含量升高;两者联合检测有助于肺癌的诊断和鉴别诊断。Objective To explore the value of detection of fragile histidine triad（FHIT） and β-tubulin in bronchoalveolar lavage fluid （BALF） in the dignosis of lung cancer. Methods The expressions of FHIT and βtubulin in BALF were detected by ELISA in 53 patients with lung cancer （group A） and 22 patients with lung benign diseases（group B）. Results The expression of FHIT in BALF was lower, but that of β-tubulin was higher, in group A than those in group B（P〈0. 05）. The expression of FHIT in BALF was lower, but that of βtubulin was higher, in the patients with lung squamous cell carcinoma and small cell lung cancer than those in those with lung adenocarcinoma （P〈0. 05）. The expressions of FHIT and β-tubulin were not significantly different among the patients with different stages of lung cancer（P〈0. 05）. Conclusion The expression of FHIT is lower and β-tubulin is higher in BALF of patients with lung cancer. Combined detection of FHIT and β-tubulin is helpful in the diagnosis and differential diagnosis of lung cancer.

关 键 词：肺癌 脆性组氨酸三联体 Β-微管蛋白 

分 类 号：R734[医药卫生—肿瘤]