HPV-16癌蛋白对肺癌细胞A549迁移和侵袭的影响及其机制  

Effects of HPV-16 oncoproteins on migration and invasion in A549 lung cancer cells and their mechanisms

作　　者：胡启英 胡启兰[2] 刘飞[3] 冯芸[3] 唐旭东[3] 

机构地区：[1]自贡市富顺县代寺区中心卫生院,四川富顺643212 [2]自贡市富顺县妇幼保健院,四川富顺643200 [3]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《现代肿瘤医学》2015年第18期2553-2557,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81372511;30872944);广东省自然科学基金项目(编号:S2012010008232)

摘　　要：目的:探讨HPV-16 E6和E7癌蛋白对肺癌细胞A549迁移和侵袭能力的影响及其机制。方法:A549细胞分别转染含HPV-16 E6、E7的EGFP质粒,采用划痕实验观察转染后不同时间的迁移能力,Transwell侵袭实验检测侵袭能力,Western blotting和实时定量PCR分别分析MMP-2和MMP-9的蛋白、mRNA表达。结果:同对照细胞相比,转染含HPV-16 E6、E7质粒的细胞迁移能力增强,在36h和48h时趋势较为明显;转染HPV-16 E6、E7组的侵袭细胞数分别为每高倍视野121.3±13.3、129.7±5.5,其显著高于对照组(P<0.01)。而且,转染HPV-16 E6、E7的细胞中MMP-2和MMP-9的蛋白和mRNA表达显著增加(P<0.01)。结论:HPV-16 E6和E7癌蛋白可增强A5 4 9细胞的迁移和侵袭能力,其机制与上调MMP-2和MMP-9表达有关。Objective：To study the effects of human papillomavirus （HPV）type 16 （HPV -16）oncoproteins on migration and invasion in A549 lung cancer cells and the underlying mechanisms.Methods：A549 cells were trans-fected with EGFP plasmids harboring HPV -16 E6 and E7 genes,respectively.After transfection,migration capability at different time was analyzed by wound healing assay.Invasion capability was detected by transwell invasion assay. The expression of MMP -2 and MMP -9 protein and mRNA was determined by Western blotting and real -time PCR,respectively.Results：Compared with control cells,the migration capability in the cells transfected with the plas-mids harboring HPV -16 E6 or E7 was enhanced,which was more obvious at 36h and 48h.The invasive cell number in the cells transfected with the plasmids harboring HPV -16 E6 and E7 was 121.3 ±13.3 and 129.7 ±5.5,respec-tively,which was much higher than that in control cells （P 〈0.01）.Furthermore,the expression of MMP -2 and MMP -9 protein and mRNA in the cells transfected with the plasmids harboring HPV -16 E6 or E7 was dramatically upregulated（P 〈0.01）.Conclusion：HPV -16 E6 and E7 oncoproteins can promote the capabilities of migration and invasion,which may be related to the upregulation of MMP -2 and MMP -9 expression.

关 键 词：人乳头瘤病毒 肺癌 迁移 侵袭 MMP-2 MMP-9 

分 类 号：R730[医药卫生—肿瘤] R735.2[医药卫生—临床医学]