乳香提取物抑制裸鼠胰腺癌生长的作用及机制研究  

The Inhibitory Effect and Mechanism of Pancreatic Cancer in Nude Mice Model with Frankincense Extract

作　　者：倪效[1] 梁晓强[1] 张静喆[1] 

机构地区：[1]上海中医药大学附属龙华医院普外科,上海200032

出　　处：《中国中西医结合外科杂志》2015年第4期376-379,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的:研究乳香提取物体内抑制胰腺癌的可能性及其机制。方法:利用人胰腺癌细胞株MIA Pa Ca-2体外扩增后种植于裸鼠皮下成瘤,将乳香提取物经裸鼠皮下注射,同时设PBS组为对照,观察肿瘤体积变化(其中乳香提取物治疗组6只,对照组5只),12 d后将肿瘤切除后测量肿瘤体积,固定瘤体后切片,通过标记磷酸化组蛋白H3(PHH3),原位末端转移酶标记技术(TUNEL)行免疫组化分析。结果:治疗组肿瘤体积为(76.67±24.11)mm3,对照组肿瘤体积为(204.80±101.19)mm3;治疗组PHH3阳性细胞数为197.5±40.4,对照组PHH3阳性细胞数为252.8±30.6;治疗组TUNEL阳性细胞数为316.7±117.3,对照组为TUNEL阳性细胞数为100.6±47.2;治疗组与对照组比较,P<0.05。结论:乳香提取物在体内可以抑制裸鼠胰腺癌的生长,促进胰腺癌细胞的凋亡。Objective To investigate the inhibitory effect and mechanism of pancreatic cancer in nude mice model with Frankincense extract. Methods MIA PaCa-2 of pancreatic cancer cell lines were implanted into nude mice subcutaneously .The nude mice were randomly assigned to receive either PBS（control） or Frankincense extract injection subcutaneously （the number of treatment group was 6, while the control group was 5）. After 12 days, tumors were removed . Tumor volume were mersured. Tumors were fixed and immunohistochemical analysis for PHH3 and TUNEL. Results The tumor size of the treatment group was （76.67 ± 24.11） mm3 while the control group was（204.80±101.19）mm3. The difference is statistically significant （p 〈 0.05）. The number of PHH3-immunoreactive cells of treatment group was （197.5±40.4）, while the control group was （252.8±30.6）. The difference is statistically significant （P〈0.05）. The number of TUNEL positive cells in treatment group was （316.7 ± 117.3）, while the control group was （100.6 ± 47.2）. The difference is statistically significant （P〈0.05）. Conclusion The Frankincense extract can inhibit the growth of pancreatic cancer in vivo of xenograft murine.

关 键 词：乳香 胰腺癌 凋亡 裸鼠 

分 类 号：Q95-33[生物学—动物学] R735.9[医药卫生—肿瘤]