转化生长因子β1抗大鼠肝脏移植排斥反应的作用及机制的实验研究  

Experimental study of effect and mechanism of transforming growth factor β1 in antirats liver transplant rejection

作　　者：张博[1] 李伟光[1] 吴波[1] 

机构地区：[1]哈尔滨医科大学附属第四医院普外科,黑龙江哈尔滨150006

出　　处：《胃肠病学和肝病学杂志》2015年第8期990-992,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：黑龙江省自然科学基金项目(GC09C409-1)

摘　　要：目的研究转化生长因子β1抗大鼠肝脏移植排斥反应的作用及机制。方法选取8~10周龄雄性大鼠230只,其中115只为SD大鼠,作为肝脏移植的供体组,115只为Wister大鼠,为肝脏移植的受体组。将进行肝脏移植Wister大鼠随机分为观察组和对照组,观察组每天腹腔注射转化生长因子β1,10 ml/d,对照组腹腔注射生理盐水,10 ml/d。连续10 d。术后3 d、7 d及14 d收集供体大鼠的血液,检测血清中IL-17、IL-12、IL-15、IL-4、IL-10的表达量,记录肝脏移植大鼠的存活天数,并进行统计分析。结果观察组大鼠存活天数为（48.3±6.8）d,对照组为（27.4±2.3）d,两组比较,差异有统计学意义（P〈0.05）;移植手术后3 d、7 d、14 d,观察组中IL-17、IL-12、IL-15的表达量明显低于对照组,差异有统计学意义（P〈0.05）,而IL-4、IL-10的表达量明显高于对照组,差异有统计学意义（P〈0.05）。结论转化生长因子β1具有抗大鼠肝脏移植排斥反应的作用,其作用机制与抑制Th1类细胞因子的表达量及促进Th2类细胞因子的表达量有关。Objective To study the effect and mechanism of transforming growth factorβ1 in anti-rat liver transplant rejection. Methods There were 230 cases 8 -10 weeks old male rats. Where 115 SD rats were used as donor liver transplantation group, 115 Wister rats were used as liver transplant recipient group. Wister rats were randomly divided into observation group and control group, the observation group were daily injected intraperitoneally transforming growth factor-β1, 10 ml/d, the control group were injected with saline, 10 ml/d. A total of 10 days. The blood of donor rats were collected after operation 3 d, 7 d and ld d, serum levels of IL-17, IL-12, IL-15, IL4, IL-10 were collected, and the survival days in liver transplantation rats were recorded. Results The survival days were （48.3 ± 6.8）d in obser- vation group and （27.4 ± 2.3 ） d in control group （P 〈 0.05 ）. After operation 3 d, 7 d and 14 d, the levels of IL-17, IL-12, IL-15 were significantly lower in observation group than those in control group （P 〈 0.05） , while the levels of IL-4 and IL-10 were significantly higher in observation group than those in control group （P 〈 0.05）. Conclusion Transforming growth factor β1 has the effect of anti-rat liver transplant rejection. The mechanism of inhibition of the ex- pression of Th1 cytokines promotes the expression of Th2 cytokines on the induction of immune tolerance.

关 键 词：转化生长因子Β1 肝脏移植 排斥反应 机制 

分 类 号：R735.35[医药卫生—肿瘤]