抑郁症患者多巴胺转运体基因可变串联重复序列多态性研究  

Variable Number of Tandem Repeat Polymorphism of DAT Gene in Patients With Depressive Disorder

作　　者：唐洪燕[1] 郭宗君[1] 李玉焕[2] 杨虎梅[1,3] 徐圣芬 赤海娇 陈娟[1,5] 

机构地区：[1]青岛大学附属医院保健科,山东省青岛市266003 [2]青岛市精神卫生中心 [3]山东省临沂市费县人民医院 [4]山东省临沂市平邑县人民医院 [5]陕西省西安兵器工业五二一医院

出　　处：《中国全科医学》2015年第24期2885-2888,共4页Chinese General Practice

基　　金：山东省自然科学基金资助项目(ZR2012HM049);山东省科技厅基金资助项目(22130109;2011YD18045);青岛市科技局基金资助项目(Kzd-03;09-1-1-33-nsh;KZJ-28);山东省保健基金资助项目(2007BZ19)

摘　　要：目的探讨抑郁症患者多巴胺转运体(DAT)基因3'非翻译区域的40 bp可变串联重复序列(VNTR)多态性。方法选取2011年10月—2014年3月就诊于青岛大学附属医院和青岛市精神卫生中心住院及门诊的抑郁症患者116例作为抑郁组,同期选取青岛大学附属医院体检健康者172例作为对照组。应用聚合酶链反应-VNTR多态性分析技术行DAT基因VNTR多态性检测。结果共检测出520、480、440、380、360 bp 5种等位基因,分别为DAT基因的VNTR多态性11、10、9、7.5、7次重复序列。6种基因型分别为:480 bp/480 bp、480 bp/440 bp、520 bp/480 bp、480 bp/380 bp、480 bp/360 bp、440 bp/440 bp。抑郁组和对照组的基因型分布和等位基因频率符合Hardy-Weinberg遗传平衡定律(抑郁组:χ2=1.380,P=0.848;对照组:χ2=0.051,P=0.990)。抑郁组与对照组基因型分布比较,差异有统计学意义(P<0.05);其中两组480 bp/480 bp和480 bp/440 bp基因型比较,差异有统计学意义(χ2=24.375,P<0.001;χ2=4.976,P<0.05)。两组等位基因频率比较,差异有统计学意义(P<0.05)。结论 DAT基因VNTR多态性可能与抑郁症的发病相关,为进一步的临床研究提供了借鉴。Objective To investigate the 40 bp variable number of tandem repeat （VNTR） polymorphism within 3＇ untranslated region of the dopamine transporter （DAT） gene of patients with depressive disorder. Methods The study enrolled 116 patients who received treatment in the Affiliated Hospital of Qingdao University and the Mental Health Center of Qingdao from October 2011 to March 2014 as depression group. Another 172 healthy people who received physical examination in the Affiliated Hospital of Qingdao University during the same period were enrolled as control group. Used PCR - VNTR technology to test the VNTR polymorphism of DAT gene. Results The study found five kinds of alleles, including 520, 480, 440, 380 and 360 bp, and they were respectively 11, 10, 9, 7. 5 and 7 repetitive sequences of VNTR polymorphism of DAT gene. The 6 genotypes were 480 bp/480 bp, 480 bp/440 bp, 520 bp/480 bp, 480 bp/380 bp, 480 bp/360 bp and 440 bp/440 bp. The distribution of genotypes and the frequency of alleles of the two groups accorded with the Hardy - Weinberg law of genetic equilibrium （depression group： X2 = 1. 380, P = 0. 848; control group： X2 = 0. 051, P = 0. 990） . The two groups were significantly different （P 〈 0. 05 ） in the distribution of genotypes; the two groups were significantly different in the distribution of 480 bp /480 bp and 480 bp/440 bp （ X2 = 24. 375, P 〈 0. 001 ; X2 = 4. 976, P 〈0. 05 ） . The two groups were also significantly different （ P 〈 0.05 ） in the frequency of alleles. Conclusion VNTR polymorphism of DAT gene may be related with the onset of depression, which provides a reference for further clinical research.

关 键 词：抑郁症 多巴胺转运体 串联重复序列 基因 多态性  单核苷酸 

分 类 号：R749.42[医药卫生—神经病学与精神病学]