bcl-2小分子干扰RNA对人子宫内膜癌RL-952细胞阿霉素敏感性的影响  

Influence of bcl-2 Small Interference RNA on the Sensibility of Endometrial Carcinoma RL-952 Cells to Doxorubicin

作　　者：周夫群[1] 黄章骞[1] 何尧[1] 

机构地区：[1]浙江省绍兴市妇幼保健院妇产科,312000

出　　处：《中国全科医学》2015年第24期2917-2921,共5页Chinese General Practice

摘　　要：目的研究靶向bcl-2小分子干扰RNA(siRNA)干扰序列对人子宫内膜癌RL-952细胞阿霉素(DOX)敏感性的影响及相关作用机制。方法 2014年5—8月,将siRNA干扰序列转染人子宫内膜癌RL-952细胞,设3个实验组:空白对照组、bcl-2 siRNA干扰组(转染bcl-2 siRNA干扰序列)及阴性对照组(转染siRNA空载序列),检测bcl-2 siRNA干扰组的转染效率和3组bcl-2表达水平。将细胞随机分成5组,分别为空白对照组、阴性对照组(转染siRNA空载序列)、干扰组(转染bcl-2 siRNA干扰序列)、DOX组(加入5μg/ml DOX)、联合组(转染siRNA干扰序列并加入5μg/ml DOX),检测细胞活力、细胞凋亡率、半胱氨酸蛋白酶(Caspase)-3、Caspase-9活性及细胞色素C水平。结果 siRNA干扰序列转染人子宫内膜癌RL-952细胞的转染效率为(94.6±12.5)%。3组bcl-2表达水平比较,差异有统计学意义(F=8.75,P<0.05)。bcl-2 siRNA干扰组bcl-2表达水平低于空白对照组和阴性对照组(q=3.99、2.87,P<0.01)。5组细胞活力比较,差异有统计学意义(F=42.52,P<0.05)。干扰组、DOX组、联合组细胞活力低于空白对照组(q=7.66、8.64、6.89,P<0.05);联合组细胞活力低于DOX组(q=6.76,P<0.01)。5组细胞凋亡率比较,差异有统计学意义(F=112.34,P<0.05)。干扰组、DOX组、联合组细胞凋亡率高于空白对照组(q=5.78、4.99、6.21,P<0.05);联合组细胞凋亡率高于DOX组(q=4.89,P<0.01)。5组Caspase-3、Caspase-9活性及细胞色素C水平比较,差异有统计学意义(P<0.05)。干扰组、DOX组、联合组Caspase-3、Caspase-9活性及细胞色素C水平高于空白对照组(P<0.05);DOX组、联合组Caspase-3活性高于阴性对照组,干扰组、DOX组、联合组Caspase-9活性高于阴性对照组(P<0.05);联合组Caspase-3活性高于干扰组,DOX组、联合组Caspase-9活性高于干扰组(P<0.05);联合组Caspase-3、Caspase-9活性及细胞色素C水平高于DOX组(P<0.05)。结论靶向bcl-2 siRNA干扰序列可以增强人子宫内膜癌RL-952细胞�Objective To research the influence of bcl-2 targeting siRNA on the sensibility of endometrial carcinoma RL-952 cells to doxorubicin （DOX） .Methods From May to August in 2014, siRNA interference sequence were transfected into endometrial carcinoma RL-952 cells.The RL-952 cells were divided into three trial groups： blank control group , bcl-2 siRNA interference group （ with transfected bcl-2 siRNA interference sequence ） and negative control group （ with transfected siRNA empty sequence ） .The transfection efficiency of bcl-2 siRNA interference group and the protein expression level of bcl-2 of the three groups were tested .The RL-952 cells were divided into five groups： control blank group , negative control group （ with transfected siRNA empty sequence ） , interference group （ with transfected bcl-2 siRNA interference sequence ） , DOC group （ added with 5μg/ml DOX） , and combination group （ with transfected siRNA interference sequence and＆amp;nbsp;5 μg/ml DOX） .Cell viability, cell apoptosis rate, the activity of Caspase-3 and Caspase-9 and cytochrome C level of the five groups were tested.Results The efficiency of transfection from siRNA interference sequence to endometrial carcinoma RL-952 cells was （94.6 ＆#177;12.5）%.The three trial groups were significantly different in the protein expression level of bcl-2 （F=8.75, P〈0.05） .The bcl-2 siRNA interference group was lower than blank control group and negative control group in the protein expression level （ q =3.99, 2.87; P 〈0.01 ） .The five groups were significantly different in cell viability （ F=42.52, P 〈0.05） .Interference group, DOC group and combination group were lower than blank control group in cell viability （q =7.66, 8.64, 6.89; P〈0.05）; combination group was lower than DOX group in cell viability （q=6.76, P〈0.01）.The five groups were significantly different in cell apoptosis rate （F=112.34, P〈0.05） .Interference group, DOX group and combination group were higher than blank

关 键 词：子宫内膜肿瘤 基因  BCL-2 RNA  小分子干扰 阿霉素 半胱氨酸蛋白酶类 Genes bcl-2 

分 类 号：R737.33[医药卫生—肿瘤]