灵芝多糖对荷膀胱癌T24细胞小鼠化疗后腹腔巨噬细胞的影响及相关免疫机制研究  

Effects of Ganoderma Lucidum Polysaccharides on Peritoneal Macrophages in Nude Mice Bearing Human Ladder Cancer T24 Cells After Chemotherapy and Related Immune Mechanism

作　　者：刘奔[1] 郭鹏荣 盛玉文[1] 傅德望[1] 王成财[1] 王朝[1] 

机构地区：[1]辽宁医学院附属第一医院泌尿外科,辽宁省锦州市121001

出　　处：《中国全科医学》2015年第24期2971-2975,共5页Chinese General Practice

基　　金：辽宁省自然科学基金资助项目(201202131)

摘　　要：目的探讨灵芝多糖对荷膀胱癌T24细胞小鼠化疗后腹腔巨噬细胞免疫功能的影响。方法 2012年5月—2014年10月建立荷膀胱癌T24细胞小鼠模型15只,采用随机数字表法分为3组:0.9%氯化钠溶液组、顺铂组、顺铂+灵芝多糖组,每组5只。分别以0.9%氯化钠溶液、0.9%氯化钠溶液、灵芝多糖灌胃18 d,以0.9%氯化钠溶液、顺铂、顺铂腹腔注射5 d。收集小鼠腹腔巨噬细胞,采用流式细胞仪检测腹腔巨噬细胞对异硫氰酸荧光素-右旋糖酐(FITC-dextran)的吞噬能力,以FITC-dexran阳性率表示;采用酶联免疫吸附试验法(ELISA)检测腹腔巨噬细胞白介素1(IL-1)和肿瘤坏死因子α(TNF-α)表达水平;采用荧光定量PCR(RT-PCR)检测腹腔巨噬细胞IL-1mRNA和TNF-αmRNA表达水平。结果顺铂+灵芝多糖组小鼠FITC-dextran阳性率较顺铂组升高4.5%;顺铂+灵芝多糖组小鼠CD11b-FITC阳性率和CD68-FITC阳性率分别较顺铂组升高11.5%和13.4%,但均未能达到0.9%氯化钠溶液组巨噬细胞中阳性率。3组小鼠腹腔巨噬细胞IL-1、TNF-α表达水平及IL-1 mRNA、TNF-αmRNA表达水平比较,差异有统计学意义(P<0.05);其中顺铂组和顺铂+灵芝多糖组小鼠腹腔巨噬细胞IL-1、TNF-α表达水平及IL-1 mRNA、TNF-αmRNA表达水平较0.9%氯化钠溶液组降低(P<0.05);顺铂+灵芝多糖组小鼠腹腔巨噬细胞IL-1、TNF-α表达水平及IL-1 mRNA、TNF-αmRNA表达水平较顺铂组升高(P<0.05)。结论灵芝多糖能够提高荷膀胱癌T24细胞小鼠腹腔巨噬细胞的吞噬作用,从而提高机体的免疫功能,其机制可能与增强IL-1和TNF-α的表达水平有关。Objective To explore the effects of ganoderma lucidum polysaccharides （ GLP） on the immune function of macrophages in mice bearing human ladder cancer T 24 cells.Methods Nude mice model bearing the human bladder cancer T 24 cells was established from May 2012 to October 2014, mice were divided into three groups by random number table method：0.9%sodium chloride solution group , cisplatin group and cisplatin ＋GLP group, there were 5 mice in each group.Mice in the above three groups received intragastric administration of 0.9% sodium chloride solution , 0.9% sodium chloride solution and GLP, respectively , and received intraperitoneal injection of 0.9% sodium chloride solution , cisplatin and cisplatin , respectively.The peritoneal macrophages were then collected , the phagocytic rate of peritoneal macrophages against FITC -dextran was further detected by flow cytometry , the levels of IL-1 and TNF-αin peritoneal macrophages were measured by ELISA, and real-time fluorescent quantitative polymerase chain reaction （ qPCR） was used to analyze the mRNA levels of IL-1 and TNF-αin peritoneal macrophages.Results The positive rates of FITC -dextran, CD11b -FITC and CD68 -FITC among＆amp;nbsp;mice in cisplatin＋GLP group were 4.5%, 11.5%and 13.4%higher than those among mice in cisplatin group , respectively , the above indicators among mice in cisplatin ＋GLP group and cisplatin group were lower than those among mice in 0.9%sodium chloride solution group , respectively.There were significant differences in levels of IL-1 and TNF-α, expression levels of IL-1 mRNA and TNF-αmRNA in peritoneal macrophages among three groups of mice （ P 〈0.05 ）; levels of IL-1 and TNF-α, expression levels of IL-1 mRNA and TNF-αmRNA in peritoneal macrophages among mice in cisplatin ＋GLP group and cisplatin group were significantly lower than those among mice in 0.9%sodium chloride solution group , respectively （ P〈0.05）; the above indicators among mice in cisplatin ＋GLP group were significantly higher than thos

关 键 词：膀胱肿瘤 灵芝 抗肿瘤联合化疗方案 巨噬细胞 

分 类 号：R737.14[医药卫生—肿瘤]