MDMA(3,4亚甲基二氧甲基苯丙胺)对小鼠睾丸细胞微核率及染色体畸变率的影响  

Effect of MDMA on Micronucleus Frequency and Chromosomal Aberration Ratio of Rats' Testicle Cells

作　　者：赵忠桂[1] 刘霜[1] 刘艳华[1] 李雅欣[1] 伍爱荣[1] 周业成[1] 

机构地区：[1]湖南环境生物职业技术学院医药学院,湖南衡阳421001

出　　处：《湖南生态科学学报》2015年第2期31-35,共5页Journal of Hunan Ecological Science

基　　金：湖南省教育厅高校科研项目(编号:12C1055)

摘　　要：通过连续20 d对雄性小鼠灌胃染毒3,4-亚甲基二氧基甲基苯丙胺(3,4-methylenedioxymethamphetamine,MDMA)后,探究MDMA对雄性小鼠睾丸组织细胞微核率及染色体畸变率的影响.将雄性小鼠随机分为MDMA低(5.0mg/kg)、中(10.0mg/kg)、高(20.0 mg/kg)三个染毒剂量组,采用生理盐水做阴性对照,每日染毒1次.于末次给药后第二天,取小鼠睾丸组织细胞,采用常规微核(micronucleus,MN)试验,检测小鼠睾丸细胞微核率的改变;同时采用染色体畸变试验(chromosomal aberration test)探究MDMA对小鼠睾丸细胞染色体畸变率的影响.微核试验结果表明MDMA中、低剂量组小鼠睾丸细胞微核率与阴性对照组比较差异无显著性(P>0.05),而高剂量与低剂量组小鼠睾丸细胞微核率及阴性对照组比较,差异均有显著性(P<0.01).染色体畸变试验结果中,MDM高、中剂量组染色体畸变率分别与阴性对照组比较差异有显著性(P<0.05),剂量组之间染色体畸变率差异也有显著性(P<0.05).结论通过微核试验与染色体畸变试验结果同时得出:在该实验剂量内MDMA高剂量组能诱导小鼠睾丸组织细胞微核率增加,高剂量组及中剂量组可以使小鼠睾丸细胞染色体畸变率增高,说明其对雄性小鼠睾丸遗传物质有一定的损伤效应.图4,表2,参10.To study the effects of 3,4-methylenedioxymethamphetamine （MDMA） on micronucleus frequency and chromosomal aberration ratio of male rats＇ testicle cells, healthy adult male rats were randomly divided into three groups. Different doses of MDMA （5, 10 and 20 mg/kg） were poisoned to the rats every days lasting for 20 days. The testicle cells were studied on the second day after being poisoned. Each rat＇s testicle micronucleus frequency and chromosomal aberration ratio were detected by using micro- nucleus test and chromosomal aberration test. The results showed that the micronucleus frequencies of rats testicle cell in low dose group was not significantly different from the control group （ P 〉 0.05 ）, while that in high dose group was significantly different from the control group and low dose group （P 〈0.01 ）. Compared with the control group, the chromosomal aberration ratios in testis of rats in high and middle dose group increased, the difference was not significant （P 〈 0.05 ）, but that in low dose group was also significantly different from the control group （ P 〈 0.05 ）. It suggested that high dose of MDMA lead to the increase of micronucleus frequency. Besides, high dose group and middle dose group could make the chromosomal aberration ratio increase. So MDMA might 2tabs. , 10refs. damage genetic material of rat testicle cells. 4figs. ,

关 键 词：MDMA 睾丸细胞 微核试验 染色体畸变试验 

分 类 号：R394.6[医药卫生—医学遗传学]