芍药苷对过氧化氢损伤的人脐静脉内皮细胞的保护作用  

The protective effects of paeoniflorin on hydrogen peroxide-damaged human umbilical vein endothelial cells

作　　者：宋献美[1] 钱丽丽[1] 梁瑞峰[2] 

机构地区：[1]河南医学高等专科学校微生物与免疫学教研室,河南郑州451191 [2]河南省中医药研究院中药研究所,河南郑州450004

出　　处：《现代预防医学》2015年第16期2914-2916,2923,共4页Modern Preventive Medicine

基　　金：国家中医药管理局重点学科建设项目(国中医药发[2009]30号);国家中医药管理局中医心病学重点学科基础研究课题(1304483)

摘　　要：目的探讨芍药苷对过氧化氢(H2O2)诱导人脐静脉血管内皮细胞(HUVEC)损伤的保护作用。方法体外培养HUVEC,以200μmol/L H2O2诱导人脐静脉血管内皮细胞损伤建立模型,同时以100、200、400μmol/L的芍药苷分别干预,显微镜下观察细胞形态,用噻唑蓝(MTT)法检测HUVEC活力,微板法检测上清液中乳酸脱氢酶(LDH)的活性及一氧化氮(NO)的含量,酶联免疫吸附法(ELISA)法检测内皮素-1(ET-1)、组织型纤溶酶原激活物(t PA)、纤溶酶原激活物抑制因子(PAI-1)的含量。结果与模型组比较,芍药苷中、高剂量组细胞活力显著升高,LDH活性显著减低、ET-1和PAI-1含量显著减少,NO和t PA显著升高(P<0.05或P<0.01)。结论芍药苷对H2O2诱导的HUVEC损伤具有明显的保护作用。Objective The study aimed to explore the protective effects of paeoniflorin on hydrogen peroxide （H202）-damaged human umbilical vein endothelial cells （HUVECs）. Methods HUVECs were cultured in vitro, and 200 μmol/L of H2O2 was used to induce HUVEC damage to establish the experimental model. 100, 200, and 400 μmol/L of paeoniflorin were used for intervention. Cellular morphology was observed under a microscope, and HUVEC viability was tested by the thiazolyl blue （MTr） method. Lactate dehydrogenase （LDH） activity and NO level in the supernatant were measured by microtiter plates. The levels of endothelin-1 （ET） -1, tectotype plasminogen activators （tPA）, and plasminogen activator inhibitors （PAI-1） were determined by enzyme-linked immunosorbent assay （ELISA）. Results Cell viability in the mid and high-dose groups significantly increased as compared with the model group. In addition, LDH activity showed a significant reduction, ET-1 and PAI-1 levels decreased significantly, and NO and tPA levels were significantly increased （P〈0.05 or P〈0.01）. Conclusion Paeoniflorin is protective against H2O2-induced HUVEC damage.

关 键 词：芍药苷 人脐静脉内皮细胞 过氧化氢 

分 类 号：R15[医药卫生—营养与食品卫生学]