多粘芽孢杆菌发酵放大工艺研究  

Scale-up Fermentation of Paenibacillus polymyxa

作　　者：周荣华[1] 崔怡宁 饶犇[1] 廖先清[1] 杨自文[1] 

机构地区：[1]湖北省农业科学院生物农药工程研究中心,武汉430064 [2]武汉中学,武汉430061

出　　处：《湖北农业科学》2015年第11期2634-2636,共3页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心项目(2013NKYJJ21);国家科技支撑计划项目(2011BAD40B01)

摘　　要：研究多粘芽孢杆菌的发酵放大工艺,探索在5t发酵罐中采用不同溶氧、不同碳源A浓度及不同种类的碳源对发酵的影响。结果表明,混用碳源B和碳源C可以较好的阻止多粘芽孢杆菌自溶,大部分菌体形成芽孢,发酵液中的芽孢数量为5亿/m L,这些研究为后面的工业化生产打下了良好的基础。We studied the scale-up fermentation of Paenibacillus polymyxa. Different dissolved oxygen, different concentration of carbon source A and different types of carbon sources on the impact of fermentation were studied at 5 tons fermenter. The results showed that the carbon B combined with carbon C can well prevent autolysis of Paenibacillus polymyxa and most bac- teria can form spores. The number of spores in the fermentation broth was 500 million/mL. All these lay a good foundation for the industrialization.

关 键 词：多粘芽孢杆菌(Paenibacillus polymyxa) 放大 自溶 

分 类 号：Q813.1[生物学—生物工程]