DPPH法测定桑籽不同极性部位抗氧化性  

Antioxidant Activity Determination of Different Polar Components Extracted from Mulberry Seeds by DPPH Method

作　　者：孟祥凯[1] 孔凡刚[1] 丁菲[1] 巩丽丽[2] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学实验中心,山东济南250355

出　　处：《食品与药品》2015年第4期255-258,共4页Food and Drug

基　　金：山东高校科技计划项目;项目编号:J12LM58;东中医药大学大学生研究训练计划(SRT)项目立项资助;项目编号2013264

摘　　要：目的研究桑籽不同极性部位的抗氧化活性。方法采用不同极性的溶剂提取桑籽,得到不同极性部位的提取物,通过测定对二苯代苦味酰肼基(DPPH)的清除能力,评价桑籽不同极性部位的抗氧化活性。结果桑籽不同极性部位的抗氧化性不同,乙酸乙酯部位EC50(半数清除率)为0.0540 mg/m L;正丁醇部位EC50为0.1521 mg/m L;石油醚部位EC50为0.3746 mg/m L;水部位EC50为0.2156 mg/m L。结论桑籽具有较强的抗氧化能力,其中乙酸乙酯部位抗氧化能力最强,石油醚部位抗氧化能力最弱。Objective To investigate the antioxidant effect in vitro of different polar components extracted from mulberry seeds. Methods Different polar components were extracted by different polar solvents. The antioxidant activities of the extracts were evaluated through determination of their capacity to scavenge diphenyl picryl hydrazinylradical （DPPH）. Results The antioxidant activities of different polar components were not identical. The EC50 （half clearance） of components extracted by ethyl acetate, n-butanol, petroleum ether and water were 0.0540, 0.1521, 0.3746 and 0.2156 mg/mL, respectively. Conclusion Mulberry seeds have relative strong antioxidant capacity. Of the different polar components, the antioxidant activity of ethyl acetate extract is the strongest and that of the petroleum ether extract is the weakest.

关 键 词：桑籽 极性部位 对二苯代苦味酰肼基 抗氧化性 

分 类 号：R285[医药卫生—中药学]