HIF-1α和NF-κB在实验性心肌缺血再灌注损伤中的表达  

The expression of HIF- 1α and NF- κB in experimental myocardial ischemia- reperfusion injury

作　　者：张明[1] 周刚[1] 戚家峰[1] 骆鹏[1] 邢宇彤[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154007

出　　处：《牡丹江医学院学报》2015年第4期4-6,共3页Journal of Mudanjiang Medical University

基　　金：佳木斯大学研究生科技创新课题资助项目(LM2014-020)

摘　　要：目的探讨雄性成年SD大鼠心肌缺血30min再灌注120min(I30minR120min)后心肌组织中缺氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)及核转录因子-κB(Nuclear transcription factor-κB,NF-κB)的表达变化。方法将30只SD大鼠分为正常组(Normal)、假手术组(Sham)和缺血30min再灌注120min组(I30minR120min),I30minR120min组结扎雄性成年SD大鼠冠状动脉左前降支,ELISA和Western blot法检测心肌组织中HIF-1α和NF-κB蛋白表达的改变。结果与假手术组相比,I30minR120min组心肌中HIF-1α和NF-κB的蛋白水平明显增加(P<0.01)。结论心肌缺血再灌注可使心肌HIF-1α和NF-κB的表达增高。Objective To study the expression of hypoxia - inducible factor 1 α （ HIF - 1α ） and nuclear transcription factor - κB（ NF -κB）in myocardial ischemia- reperfusion injury of adult male SD rats. Methods 30 adult male SD rats were randomly divid- ed into 3 groups： normal group, sham group and I30minR12Omin group. SD rats in I30minR12Omin group were subjected to 30 -min myocardial ischemia by ligation of the left anterior descending coronary artery and 120 - min reperfusion. The expressions of HIF-1α and NF-κB were measured in myocardium by Western blot and ELISA. Results Compared with the sham group, the levels of both HIF-1α and NF-κB were increased in the ischemic region of myoeardium in ischemia 30 min and reperfasion 120 min. Conclusion The expression of HIF-1αandNF-κB in myocardium was increased in myocardial ischemia reperfusion injury.

关 键 词：心肌 缺血再灌注损伤 HIF-1Α NF-ΚB 

分 类 号：R33[医药卫生—人体生理学]