瘦素通过上调IL-18表达促进人乳腺癌MCF-7细胞迁移及侵袭  

Leptin promotes migration and invasion of human breast cancer MCF-7 cells by up-regulating the expression of IL-18

作　　者：魏兰[1] 苏敏[1] 黄云秀[1] 钟昌莉 王念[1] 季飞虎[1] 陈婷梅[1] 

机构地区：[1]重庆医科大学教育部临床检验诊断学重点实验室,重庆400016

出　　处：《肿瘤》2015年第8期827-833,共7页Tumor

基　　金：国家自然科学基金资助项目(编号:81272544);重庆市自然科学基金计划项目(编号:cstc2012jj A10011)

摘　　要：目的 :探讨瘦素(leptin)对乳腺癌MCF-7细胞迁移和侵袭能力的影响及其可能的分子作用机制。方法 :用leptin(200 ng/m L)处理乳腺癌MCF-7细胞后,采用划痕愈合实验和Transwell侵袭实验分别检测MCF-7细胞的迁移及侵袭能力,采用实时荧光定量PCR、蛋白质印迹法和ELISA法检测白细胞介素18(interleukin-18,IL-18)的表达水平。用leptin(200 ng/m L)和IL-18结合蛋白(IL-18 binding protein,IL-18bp)(10 ng/m L)同时处理乳腺癌MCF-7细胞后,再采用划痕愈合实验和Transwell侵袭实验分别检测MCF-7细胞迁移及侵袭能力的变化。结果 :Leptin可促进乳腺癌MCF-7细胞的迁移及侵袭(P值均<0.05),上调MCF-7细胞中IL-18 m RNA及蛋白的表达水平(P值均<0.05),促进IL-18分泌(P<0.05)。IL-18bp与细胞分泌的IL-18结合后,leptin促进乳腺癌MCF-7细胞迁移及侵袭的能力被明显抑制(P值均<0.05)。结论 :Leptin可促进乳腺癌MCF-7细胞的迁移及侵袭,其机制可能与促进IL-18表达上调相关。Oblective： To investigate the effects of leptin on migration and invasion of human breast cancer MCF-7 cells, and to explore its possible molecular mechanism.Methods： After treatment with 200 ng/mL leptin, the migration and invasion abilities of MCF-7 cells were detected by scratch wound healing assay and Transwell invasion test, respectively. The expression level of interleukin-18 （IL-18） in MCF-7 cells after leptin intervention was detected by real-time fluorescent quantitative-PCR,Western blotting and enzyme-linked immunosorbent assay （ELISA）. Moreover, the migration and invasion abilities of MCF-7 cells after treatment with combination of 200 ng/mL leptin and 10 ng/mL IL-18 binding protein （IL-18bp） were detected by scratch wound healing assay and Transwell invasion test.Results： Leptin promoted the migration and invasion of MCF-7 cells （both P 〈 0.05）, up- regulated the expression levels of IL-18 mRNA and protein （both P 〈 0.05）, and promoted the secretion level of IL-18 in MCF-7 cells （P 〈 0.05）, However, IL-18bp could inhibit the effects of leptin on migration and invasion of MCF-7 cells （both P 〈 0.05）.Conclusion： Leptin promotes the migration and invasion of MCF-7 cells, and its mechanism may be related to the up-regulation of IL-18 expression.

关 键 词：乳腺肿瘤 瘦素 白细胞介素18 细胞迁移分析 基因表达调控 肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]