静脉注射骨髓间充质干细胞条件培养基治疗脑缺血再灌注损伤  

Conditioned medium of bone marrow mesenchymal stem cells via intravenous injection to treat cerebral ischemia-reperfusion injury

作　　者：陈峰[1] 刘斌[1] 明圆圆[1] 周苏芹 沈霞[1] 花放[1] 崔桂云[1] 岳炫烨[1] 昝坤[1] 叶新春[2] 

机构地区：[1]徐州医学院,江苏省徐州市221002 [2]徐州医学院附属医院神经内科,江苏省徐州市221002

出　　处：《中国组织工程研究》2015年第28期4544-4548,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年项目(81201025);江苏省自然科学基金面上项目(BK20131118);江苏省六大高峰人才项目(WSN-081)资助~~

摘　　要：背景:研究证实骨髓间充质干细胞对于脑缺血再灌注损伤有积极的治疗作用,但是经尾静脉给予骨髓间充质干细胞条件培养基的研究报道较少。目的:观察经尾静脉注射骨髓间充质干细胞条件培养基对大鼠脑缺血再灌注损伤后神经功能恢复的影响。方法:体外分离培养大鼠骨髓间充质干细胞,第2、3代骨髓间充质干细胞培养融合达90%时弃培养基,DMEM培养基无血清培养18 h,收集的培养液即为骨髓间充质干细胞条件培养基。将36只右侧大脑中动脉缺血再灌注模型大鼠随机分为3组:对照组,单纯培养基对照组和条件培养基组,分别于术后2,24,48 h经尾静脉给予生理盐水、DMEM培养基、条件培养基(10 m L/kg)。结果与结论:术后2 h各组大鼠神经功能缺损评分差异无显著性意义(P>0.05)。术后第1,3,5天对照组与单纯培养基组大鼠神经功能缺损评分差异无显著性意义,条件培养基组大鼠神经功能评分明显低于对照组和单纯培养基组,差异有显著性意义(P<0.05)。术后5 d条件培养基组较对照组、单纯培养基组脑组织含水量显著减少(P<0.05),对照组和单纯培养基组相比差异无显著性意义(P>0.05)。结果说明尾静脉注射骨髓间充质干细胞条件培养基可以减轻大鼠脑缺血再灌注损伤后脑水肿程度,改善神经功能。BACKGROUND：Large numbers of experimental data have confirmed that bone marrow mesenchymal stem cel s have a positive therapeutic effect on cerebral ischemia-reperfusion injury, but there are few reports about intravenous administration of bone marrow mesenchymal stem cel conditioned medium in the treatment of stroke. OBJECTIVE：To investigate the effects of the conditioned medium of rat bone marrow mesenchymal stem cel s on the recovery of neurological function in rats after cerebral ischemia-reperfusion injury. METHODS：The bone marrow mesenchymal stem cel s were isolated from rat bone marrow. When cel s at passage 2 or 3 reached 90%confluence, the original culture medium was removed. Then the cel s were cultured in serum-free DMEM for 18 hours. After that, the culture solution was col ected as the conditioned medium of rat bone marrow mesenchymal stem cel s. Adult rats were subjected to 2 hours of right middle cerebral arteryocclusion. Ischemia-reperfusion injury rats were randomly assigned to three groups：control group, simple culture medium group and conditioned medium group, and respectively given injection of normal saline, DMEM, conditioned medium （10 mL/kg） via the tail vein at 2, 24, 48 hours after operation. RESULTS AND CONCLUSION：There was no difference in the behavioral tests among the three groups at postoperative 2 hours （P〉0.05）. Compared with the control and simple culture medium group, neurological impairment was significantly improved in the conditioned medium group at postoperative 1, 3, 5 days （P〈0.05）, but there was no significant difference between the control and simple culture medium groups. At postoperative 5 days, brain edema was significantly eased in the conditioned medium group in comparison to the control and simple culture medium groups （P〈0.05）, and there was also no difference between the latter two groups （P〉0.05）. These results suggest that rat bone marrow mesenchymal stem cel s-conditioned medium via intravenous administration can signific

关 键 词：干细胞 移植 骨髓间充质干细胞 条件培养基 经尾静脉 缺血再灌注 脑水肿 神经保护 大脑中动脉 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]