脐血间充质干细胞移植治疗帕金森病的可行性  

Umbilical cord blood mesenchymal stem cell transplantation for Parkinson's disease:a feasibility study

作　　者：刘磊[1] 冯德朋[2] 陈燕[1] 赵修敏[1] 冯肖亚[1] 葛汝村[1] 郇英[1] 吕涌涛 

机构地区：[1]山东省交通医院神经内科,山东省济南市250031 [2]聊城市人民医院神经内科,山东省聊城市252000

出　　处：《中国组织工程研究》2015年第28期4567-4571,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究证实干细胞在体内体外均可被诱导分化成多巴胺能神经元,这为干细胞移植治疗帕金森病提供了理论基础。目的:探讨脑内移植脐血间充质干细胞治疗帕金森病模型大鼠的可行性及作用机制。方法:SD大鼠脑内注射6-羟基多巴建立帕金森病模型,将22只造模成功大鼠随机分为脐血间充质干细胞移植组12只和对照组10只,脑内分别注射脐血间充质干细胞悬液和磷酸盐缓冲液。细胞移植后第1-8周,每周腹内注射阿朴吗啡观察大鼠旋转行为,并于第2,8周取大鼠纹状体和黑质部分行免疫组织化学染色。结果与结论:1脐血间充质干细胞移植组大鼠转圈次数随着时间延长逐渐下降,而对照组大鼠转圈次数没有明显改变,且移植后3-8周两组旋转圈数差异有显著性意义(P<0.05)。2移植后2周时,脐血间充质干细胞移植组大鼠纹状体针道内及附近有酪氨酸羟化酶阳性细胞存在;对照组大鼠的纹状体针道处无外源性细胞存在。移植后8周时,脐血间充质干细胞移植组鼠纹状体针道内仍有细胞存活,并有酪氨酸羟化酶阳性细胞存在,对照组大鼠纹状体处无酪氨酸羟化酶阳性细胞表达。结果表明脐血间充质干细胞移植后可在脑内存活并且表达酪氨酸羟化酶蛋白,且能改善帕金森病模型大鼠的行为学异常。BACKGROUND：Stem cel s can be induced to differentiate into dopaminergic neurons in vivo and in vitro, which provides a theoretical basis for stem cel transplantation in the treatment of Parkinson’s disease OBJECTIVE：To explore the feasibility and mechanism of intracerebral transplantation of umbilical cord blood mesenchymal stem cel s for treatment of Parkinson’s disease rats. METHODS：Intracerebral injection of 6-hydroxydopamine was used to make Parkinson’s disease models in SD rats. Twenty-two model rats were randomized into cel transplantation group （n=12） and control group （n=10） and respectively injected intracerebral y with umbilical cord blood mesenchymal stem cel suspension and PBS. At 1-8 weeks after cel transplantation, intra-abdominal injection of apomorphine was performed every week to observe the rotation behaviors of rats;at the 2nd and 8th weeks, rat’s striatum and substantia nigra were taken for immunohistochemistry staining. RESULTS AND CONCLUSION：The rotation behaviors were gradual y decreased with time in the cel transplantation, but had no changes in the control group. At 3-8 weeks after transplantation, there were significant differences in the rotation behaviors between the two groups （P〈0.05）. At 2 weeks after transplantation, tyrosine hydroxylase-positive cel s were found within and around the striatum of the cel transplantation group;but there were no exogenous cel s in the control group. At 8 weeks after transplantation,there were stil active cel s and tyrosine hydroxylase-positive cel s in the striatum of cel transplantation group, and there was no tyrosine hydroxylase expression in the striatum of the control group. These findings suggest that transplanted umbilical cord blood mesenchymal stem cel s can survive in the brain that are positive for tyrosine hydroxylase, which can improve the behavior abnormalities of Parkinson’s disease rats.

关 键 词：干细胞 移植 脐血间充质干细胞 帕金森病 大鼠 6-羟基多巴 阿朴吗啡 旋转行为 

分 类 号：R394.2[医药卫生—医学遗传学]