ADAM17对前列腺癌细胞TGF-α、EGFR的表达影响  

Effects of ADAM17 on TGF-α and EGFR in prostate cancer cells

作　　者：姜颖[1] 刘紫君[2] 林平[3] 林峰[3] 于晓光[3] 

机构地区：[1]黑龙江中医药大学基础医学院,哈尔滨150000 [2]黑龙江中医药大学附属第一医院 [3]哈尔滨医科大学基础医学院

出　　处：《山东医药》2015年第26期1-4,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30772173)

摘　　要：目的探讨前列腺癌DU145细胞中解聚素—金属蛋白酶17(ADAM17)与转化生长因子α(TGF-α)活化的关系。方法培养前列腺癌DU145细胞,将细胞分为对照组、NC组、ADAM17-siRNA组、联合组。ADAM17-siRNA组、联合组均转染ADAM17 siRNA,NC组转染非ADAM17相关的阴性序列siRNA,对照组不转染。联合组转染72 h后加入TGF-α20μg/L干预。待NC组、ADAM17-siRNA组转染72 h后,收集对照组、NC组、ADAM17-siRNA组细胞,采用Western blot法检测ADAM17、表皮生长因子受体(EGFR)、磷酸化EGFR(p EGFR)蛋白表达,采用ELISA法检测TGF-α蛋白水平。取对照组、ADAM17-siRNA组及联合组TGF-α干预15 min后的上清液,采用Western blot法检测EGFR、p EGFR蛋白表达。结果ADAM17-siRNA组ADAM17、p EGFR、TGF-α蛋白均低于对照组及NC组(P均<0.05)。对照组、NC组、ADAM17-siRNA组间EGFR表达差异无统计学意义(P均>0.05)。联合组p EGFR蛋白表达高于对照组及ADAM17-siRNA组(P均<0.05)。对照组、ADAM17-siRNA组、联合组间EGFR蛋白表达差异无统计学意义(P均>0.05)。结论前列腺癌DU145细胞中ADAM17的表达与TGF-α及EGFR有关,ADAM17可能通过活化TGF-α进而激活p EGFR表达。Objective To investigate the relationship between a disintegrin and metalloprotease 17 （ADAM17） and transfor-ming growth factor-α（TGF-α） activation in prostate cancer cells DU145.Methods We cultured the prostate cancer DU145 cells. Then, the cells were divided into the control group, NC group, ADAM17-siRNA1 group and the combination group.ADAM17 siRNA was transfected into both the ADAM17-siRNA1 group and combination group.The NC group was transfected with non-DAM17 related sequence siRNA.After the combination group was transfected for 72 h, 20 g/L TGF-αwas added.After 72-hour transfection, the cells of the control group, NC group and ADAM17-siRNA1 group were collected.Western blotting was used to detect the protein ex-pression of ADAM17, epidermal growth factor receptor （EGFR） and pEGFR.ELISA was used to detect the level of TGF-αprotein. We collected the supernatant intervened for15 mins of TGF-αfrom the control group, ADAM17-siRNA1 group and the combination group, and Western blotting was used to detect the protein expression of EGFR and pEGFR.Results The protein expression of AD-AM17, pEGFR and TGF-αin the ADAM17-siRNA1 group was lower than that of the control group （all P〈0.05）.There was no sig-nificant difference in EGFR expression between the control group, NC group and ADAM17-siRNA1 group （P〉0.05）.The expression of pEGFR in the combination group was higher than that in the control group and the ADAM17-siRNA1 group （P〈0.05）.There was no significant difference in the EGFR expression between the control group, ADAM17-siRNA1 group and the combination group （P〉0.05）.Conclusion The expression of ADAM17 in prostate cancer DU145 cells is correlated with the activation of TGF-αand EG-FR, and ADAM17 activates pEGFR expression by activating TGF-α.

关 键 词：前列腺癌 解聚素-金属蛋白酶17 转化生长因子Α 表皮生长因子受体 

分 类 号：R737.25[医药卫生—肿瘤]