褐藻多糖对乳腺癌细胞活性的影响及机制探讨  

Effects of Fucoidan on cell viability of breast cancer and its mechanism

作　　者：郝剑[1] 吴雄志[1] 

机构地区：[1]天津医科大学肿瘤医院国家肿瘤临床医学研究中心天津市"肿瘤防治"重点实验室,天津300060

出　　处：《山东医药》2015年第30期5-7,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81173376);新世纪优秀人才基金资助项目(NCET-11-1068)

摘　　要：目的观察褐藻多糖对人乳腺癌细胞株MCF-7、MDA-MB-231细胞活性的影响,并探讨其作用机制。方法将对数生长期的MCF-7、MDA-MB-231细胞(观察A组、B组)分别加入100、200μg/m L褐藻多糖2 m L,对照A组、B组均加入等体积的DMEM培养基2 m L。用台盼蓝染色实验测算细胞增殖抑制率,用流式细胞仪检测细胞周期和凋亡细胞,用Western blot法检测细胞Caspase-8及磷酸化细胞外调节蛋白激酶(p-Erk)、Erk表达。结果随着褐藻多糖浓度增加、作用时间延长,观察A组、B组细胞增殖抑制率逐渐升高(P均<0.05);观察A组、B组G0/G1期细胞比例升高、S期及G2期细胞比例降低,细胞凋亡率增高,褐藻多糖浓度高者变化更明显,P均<0.05;观察A组、B组细胞Caspase-8表达升高、p-Erk表达下降,褐藻多糖浓度高者变化更明显,P均<0.05。结论褐藻多糖可抑制MCF-7、MDA-MB-231细胞增殖,阻滞细胞周期于G0/G1期,促进细胞凋亡;该作用可能是通过上调细胞Caspase-8表达、下调p-Erk表达水平实现。Objective To observe the effects of Fucoidan on cell viability of breast cancer cell lines MCF -7 and MDA-MB-231 and to investigate its possible mechanism .Methods The breast cancer cell lines MCF-7 and MDA-MB-231 in the logarithmic phase （ observation group A and B ） were respectively treated with 2 mL Fucoidan （ 100 ug/mL and 200 ug/mL） .The control group A and B were treated with the same volume of DMEM culture medium .The cell proliferation inhi-bition rate was detected by Trypan blue staining , cell cycle and apoptosis cells were analyzed by flow cytometry , and the expression of Caspase-8, phosphorylation Erk （ p-Erk） and total Erk was detected by Western blotting .Results With the increased Fucoidan concentration and the prolonged time , the cell proliferation inhibition rates of the observation groups A and B were increased （all P〈0.05）, the cell proportion in the G0/G1 phase of the observation groups A and group B was increased, the cell proportion in the S phase and G2 was reduced, the apoptosis rate was increased, and the changes in the high concentration Fucoidan group were more significant （all P〈0.05）.The expression of Caspase-8 was increased and the Erk expression was decreased in the observation groups A and B , and the changes in the high concentration Fucoidan group were more significant （all P〈0.05）.Conclusion Fucoidan could inhibit the proliferation of MCF-7 and MDA-MB-231 cell lines, arrest the breast cells in G0/G1 phase, and also promote the apoptosis.This mechanism may be achieved by activating the expression of Caspase-8 and down-regulating the p-Erk expression .

关 键 词：褐藻多糖 乳腺癌 细胞周期 细胞凋亡 半胱氨酸天冬氨酸蛋白酶8 细胞外调节蛋白激酶 

分 类 号：R737.9[医药卫生—肿瘤]