青少年正畸治疗早期牙龈卟啉单胞菌及其毒力因子牙龈蛋白K的变化研究  

Changes of porphyromonasgingivalis and its virulence factor gingipain K during orthodontic treatment

作　　者：朱明珍[1] 韩志强[1] 张玉杰[1] 肖水清[1] 张丽[1] 

机构地区：[1]济南市口腔医院正畸科,山东济南250001

出　　处：《口腔医学》2015年第8期623-627,共5页Stomatology

基　　金：济南市科技局企业创新计划(201201057)

摘　　要：目的分析牙龈卟啉单胞菌及其毒力因子牙龈蛋白K在青少年正畸治疗早期的变化。方法随机收集青少年正畸牙龈正常者45例,分别取矫治器戴入前,戴入后1、2、3、6个月龈沟液标本,应用16S r DNA PCR技术检测各样本中的牙龈卟啉单胞菌及其毒力因子牙龈蛋白K;采用SPSS17.0软件包对数据进行统计分析,牙龈卟啉单胞菌及牙龈蛋白K各组间检出率比较用χ2检验;牙龈卟啉单胞菌、牙龈蛋白K的检出率与牙龈炎症之间的关系用Spearman等级相关分析。结果牙龈卟啉单胞菌在矫治前与戴入后1个月有显著性差异(P<0.05),与2个月、3个月之间有极显著性差异(P<0.01);戴入后1个月与2个月之间有显著性差异(P<0.05);牙龈蛋白K在矫治器戴入前与戴入后1个月、6个月之间无显著性差异,与戴入后2个月、3个月之间有显著性差异(P<0.05);从第2个月开始牙龈卟啉单胞菌与牙龈蛋白K的检出率下降,到6个月时检出率已基本接近矫治器戴入前。结论青少年正畸治疗过程中在矫治器戴入早期可引发牙龈卟啉单胞菌及牙龈蛋白K的增加,出现牙龈炎症反应,当增加到一定时间即2个月后开始逐渐下降,到6个月时已基本接近矫治器戴入前。Objective To explore the changes of porphyromonasgingivalis and its virulence factor gingipain K during orthodontic treatment. Methods Totally 45 orthodontic healthy teenagers were randomly chosen from the orthodontics department. Givgival crevicular fluid before orthodontic treatment,in the first,second,third and sixth month after treatment were collected simultaneouslyat each time point. 16 S r DNA PCR technique was used to detect porphyromonasgingivalis and its virulence factor gingipain K. SPSS 17. 0 software package was used to analyze the data. The detection rates of porphyromonasgingivalis and gingipain K between groups were compared by χ^2. The correlation of the detection rates and gingival inflammation was analyzed by Spearman. Results Porphyromonasgingivalis before treament was significantly different form that in the first month after treatment（ P〈0. 05）,and demonstrated extremely significant difference from that in the second and third month（ P〈0. 01）. Difference was also found between the first month after treatment and the second month after treatment. As for gingipain K,there was no difference before treatment,the first month after treatment and the sixth month after treatment,while statistical difference was found between the second month after treatment and the third month after treatment（ P〈0. 05）. Since the third month after treatment,the rate of porphyromonasgingivalis and gingipain K had decreased,and in the sixth month after treatment,it approached the level before treatment. Conclusions During the early process of orthodontic treatment,porphyromonasgingivalis and gingipain K may increase and can lead to inflammatory reaction. Started from the second month after treatment,it began to decline and it basically approached the level before treatment in the sixth month.

关 键 词：正畸治疗 牙龈卟啉单胞菌 牙龈蛋白K 牙龈炎 

分 类 号：R783.5[医药卫生—口腔医学]