不同波形电针对PCPA致失眠大鼠下丘脑5-HT和GLu、GABA含量的影响  

Effects of Different Waveform Electroacupuncture on the 5-HT,Glu and CABA Contents of the Hypothalamus in Rats with PCPA-induced Insomnia

作　　者：蒋洁[1,2] 赵百孝[2] 哈略 赵华[1] 

机构地区：[1]新疆医科大学中医学院，乌鲁木齐 830054 [2]北京中医药大学，北京 100029

出　　处：《上海针灸杂志》2015年第7期678-681,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：新疆医科大学科研创新基金项目(XJC201212)

摘　　要：目的观察电针不同波形刺激对氯苯丙氨酸(PCPA)致失眠大鼠下丘脑5-羟色胺(5-HT)及谷氨酸(Glu)、?-氨基丁酸(GABA)含量的影响,探讨电针不同波形刺激对失眠的效应差异及作用机制。方法将50只SD大鼠随机分为正常组10只、模型组10只和电针组30只。电针组再随机分为电针1组、电针2组和电针3组,每组10只。除正常组外,其余各组大鼠均用PCPA建立大鼠失眠模型。各电针组均进行电针治疗,其中电针1组采用疏波,电针2组采用密波,电针3组采用疏密波。治疗6 d后,脱颈处死各组大鼠,采用酶联免疫吸附法测定下丘脑中5-HT、Glu、GABA含量。结果与空白组比较,模型组大鼠脑内5-HT含量明显减少(P<0.01);与模型组比较,3组电针组大鼠脑内5-HT含量均增加(P<0.01)。电针2组和电针3组大鼠脑内5-HT含量与电针1组比较,差异均具有统计学意义(P<0.05,P<0.01)。电针3组大鼠脑内5-HT含量与电针2组比较,差异具有统计学意义(P<0.01)。与空白组比较,模型组大鼠大脑皮质Glu、GABA含量均明显增高(P<0.01),Glu/GABA比值增大(P<0.01);与模型组比较,3组电针组大鼠大脑皮质Glu、GABA含量均显著减少(P<0.01),Glu/GABA比值减小(P<0.01)。电针2组和电针3组大鼠大脑皮质Glu、GABA含量与电针1组比较,差异均具有统计学意义(P<0.05,P<0.01)。电针3组大鼠大脑皮质Glu、GABA含量与电针2组比较,差异均具有统计学意义(P<0.01)。结论电针可以调节失眠大鼠下丘脑内单胺类神经递质5-HT和氨基酸类神经递质Glu及GABA含量,减少脑内氨基酸毒性,从而发挥治疗失眠的作用。其中疏波电针治疗效果优于密波和疏密波。Objective To investigate the effects of electroacupuncture of different stimulation waveforms on the 5-hydroxytryptamine （5-HT）, glutamic acid （Glu） and gamma-aminobutyric acid （CABA） contents of the hypothalamus in rats with para-chlorophenylalanine （PCPA）-induced insomnia and explore the difference in the effects and the mechanism of its action. Methods Fifty SD rats were randomly allocated to a normal group of 10 rats, a model group of 10 rats and an electroacupuncture group of 30 rats. The electroacupuncture group was then randomly divided to electroacupuncture groups 1, 2 and 3, 10 rats each. A rat model of insomnia was made using PCPA in all groups of rats except the normal group. All the electroacupuncture groups received electroacupuncture treatment. Of them, electroacupuncture group 1 received sparse wave treatment; electroacupuncture group 2, dense wave treatment;electroacupuncture group 3, sparse-dense wave treatment. After 6 days of treatment, every group of rats was sacrificed by cervical dislocation. The 5-HT, Glu and CABA contents of the hypothalamus were measured by enzyme-linked immunosorbent assay. Results The 5-HT content of the brain decreased significantly in the model group of rats compared with the blank group （P〈0.01） and increased significantly in electroacupuncture group 3 of rats compared with the model group （P〈0.01）. There was a statistically significant difference in brain 5-HT content between electroacupuncture group 2 or 3 of rats and electroacupuncture group 1 （P〈0.05,P〈0.01） and between electroacupuncture groups 3 and 2 of rats （P〈0.01）. The Glu and CABA contents of the cerebral cortex and the ratio of Glu/CABA increased significantly in the model group of rats compared with the blank group （both P〈0.01） and decreased significantly in electroacupuncture group 3 of rats compared with the model group （both P〈0.01）. There were statistically significant differences in the Glu and CABA contents of the cerebral cortex between electr

关 键 词：针刺疗法 电针 失眠症 氯苯丙氨酸 5-羟色胺 谷氨酸 Y-氨基丁酸 大鼠 波形 

分 类 号：R2-03[医药卫生—中医学]