植物乳杆菌来源的α-葡萄糖苷酶抑制剂的分离纯化  

Purification of alpha-glucosidase inhibitors from Lactobacillus plantarum

作　　者：张欢欢[1,2] 韩瑨[1] 游春苹[1] 刘振民[1] 郭本恒[1,2] 吴正钧[1] 

机构地区：[1]乳业生物技术国家重点实验室光明乳业股份有限公司,上海200436 [2]上海海洋大学食品学院,上海201306

出　　处：《应用化工》2015年第8期1417-1420,1426,共5页Applied Chemical Industry

基　　金："十二五"国家科技支撑计划课题(2013BAD18B01);"十二五"国家863项目(2011AA100901)

摘　　要：通过薄板层析、硅胶柱层析等方法对植物乳杆菌ST-III发酵豆浆中的α-葡萄糖苷酶抑制剂进行了分离纯化,通过质谱鉴定确定其分子量。结果表明,经分离纯化,得到两种α-葡萄糖苷酶抑制剂组分F1和F2,其分子量分别为271.1和255.1,对α-葡萄糖苷酶抑制作用的IC50分别为0.41 mg/m L和0.65 mg/m L。结果显示植物乳杆菌ST-III发酵豆浆是一种优良的α-葡萄糖苷酶抑制剂来源,具有潜在的抗高血糖作用。Alpha-glucosidase inhibitors were isolated and purified by silica gel thin layer and silica gel column choromatography. The molecular weights of the α-GI was identified by mass spectrometry. The resuits showed that two alpha-glucosidase inhibitors, namely F1 and F2 were isolated from the fermented soymilk by Lb. plantarum ST-Ⅲ. The molecular weights of F1 and F2 determined by mass spectrometry were 271.1 and 255.1 respectively, while the half inhibition concentration （IC50） to alpha-glucosidase of F1 and F2 were 0.41 mg/mL and 0. 65 mg/mL, respectively. The results suggested that fermented soymilk by Lb. plantarum ST-Ⅲ was an excellent resource for natural alpha-glucosidase inhibitors, which indicating the popential role of the probiotic strain in antihyperglycemia.

关 键 词：Α-葡萄糖苷酶抑制剂 植物乳杆菌ST-Ⅲ 纯化 半数抑制浓度 

分 类 号：TQ2[化学工程—有机化工] TQ46