机构地区:[1]新疆农业大学动物科学学院,乌鲁木齐830052
出 处:《新疆农业大学学报》2015年第2期120-125,共6页Journal of Xinjiang Agricultural University
基 金:新疆维吾尔自治区高技术研究发展项目(201111115)
摘 要:选取5月龄新疆吐鲁番黑羊、阿勒泰羊各6只公羊,对吐鲁番黑羊及阿勒泰羊的背最长肌肌肉品质、肌纤维特性进行比较,并对肌纤维特性与肌肉品质的相关性进行了研究。通过制作背最长肌的冰冻组织切片,采用ATP酶染进行肌纤维的分型,利用CellSens Standard图像处理系统分析肌纤维类型,测定不同肌肉组织红白肌肌纤维数量和面积,利用SPSS17.0软件对吐鲁番黑羊及阿勒泰羊背最长肌肌纤维与部分肉品质进行分析。吐鲁番黑羊和阿勒泰羊Ⅰ型肌纤维数量和直径之间差异均极显著(P<0.01),Ⅱb型肌纤维直径之间差异显著(P<0.05)。两个品种Ⅰ型和Ⅱb型肌纤维横截面积之间差异均极显著(P<0.01)。阿勒泰羊和吐鲁番黑羊背最长肌剪切力、水分含量、肌内脂肪含量之间均差异显著(P<0.05)。吐鲁番黑羊背最长肌Ⅰ型肌纤维直径与水分含量呈显著负相关(P<0.05);Ⅱb型肌纤维横截面积与剪切力呈极显著负相关(P<0.01);Ⅰ型肌纤维横截面积与肌内脂肪含量呈显著负相关(P<0.05)。阿勒泰羊背最长肌Ⅱb型肌纤维直径与剪切力呈极显著正相关(P<0.01);Ⅱb型肌纤维横截面积与水分含量呈显著正相关(P<0.05)。阿勒泰羊各型肌纤维直径均大于吐鲁番黑羊肌纤维直径,阿勒泰羊肉剪切力大于吐鲁番黑羊;肌纤维直径与剪切力呈正相关,与肉质呈负相关。吐鲁番黑羊背最长肌中Ⅰ型肌纤维含量多,Ⅰ型肌纤维直径小,数量多,嫩度好。吐鲁番黑羊背最长肌部分肌肉品质优于阿勒泰羊的背最长肌。Five month age Turpan black sheep and Altay sheep in Xinjiang were used as test materials, their longissimus muscle quality fiber characteristics were compared and relativity between their muscle fi-ber characteristics and muscle quality were investigated.The longissimus dorsi muscle frozen tissue sections were stained with the enzyme ATP type of muscle fiber.The muscle fiber type were analyzed by using CellSens Standard image system.The muscle fiber and area of different muscle tissue were determined.The diameters of muscle fiber and meat qualty of longissimus dorsi muscle fiber in Turpan black sheep and Al-tay sheep were analyzed by SPSS17.0 software.The differences between number of type Ⅰ muscle fiber number and meat quality of Turpan black sheep and Altay sheep were signigicant.There were significant differences between Turpan black sheep and Altay sheep the number ofⅠtype muscle fibers (P 〈0.01 );The difference of Ⅰtype muscle fiber diameter between the two localsheep was significant (P 〈0.01);The diameter ofⅡb type muscle fiber have significant difference (P 〈0.05).There were significant difference between the two local sheep cross-sectional area of type Ⅰ and Ⅱ b type muscle fiber (P 〈 0 .0 1 ).There were significant difference of longissimus dorsi muscle shear stress and water content between Altay sheep and Turpan black sheep (P 〈0.05);Difference of intramuscular fat content between the two local sheep was significant (P 〈0.05).Turpan black sheep longissimus dorsi muscle type Ⅰ muscle fiber diameter was negatively correlated with water content (P 〈0.05);Ⅱb type muscle fiber cross-sectional area and shear stress was a significant negative correlation (P 〈0.01);type Ⅰmuscle fiber cross-sectional area and intra-muscular fat content was negatively correlated (P 〈0.05).Ⅱb type muscle fiber diameter and shear stress were significant positive correlation in Altay sheep longissimus dorsi muscle (P 〈0.01);Ⅱb type of mus-cle fiber c
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