贮藏温度及时间对核桃品种花粉生活力的影响  

Effect of Different Storage Temperature and Time on Pollen Viability of Walnut Cultivars

作　　者：刘杜玲[1] 张博勇[1] 彭少兵[1] 吕平会[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《北方园艺》2015年第16期7-10,共4页Northern Horticulture

基　　金：陕西省农业攻关资助项目(2014K01-10-01);西北农林科技大学基本科研业务费专项资助项目(QN2013080)

摘　　要：以＂香玲＂、＂西林3号＂、＂西洛2号＂、＂强特勒＂和＂哈特勒＂5个核桃品种的花粉为试材,用TTC染色法和离体培养法测定了不同贮藏温度及时间下花粉的生活力。结果表明：5个核桃品种花粉在室温（20℃）下贮藏生活力最低,在3℃和-20℃下贮藏生活力较高;随贮藏时间延长花粉生活力显著下降。用TTC染色法测得的花粉生活力显著高于离体培养法,2种测定方法之间具有极显著的相关性。＂香玲＂、＂西林3号＂、＂西洛2号＂和＂强特勒＂花粉适于在3℃下贮藏,＂哈特勒＂花粉适于在-20℃下贮藏,贮藏时间为6～9d。Pollen vitality of 5 walnut cultivars ‘Xiangling＇,‘Xilin 3＇,‘Xiluo 2＇,‘Chandler＇and ‘Hartley＇were determined by TTC staining and in vitro culture method under different storage temperatures and time.The results showed that,the pollen viability of 5walnut cultivars were the lowest at room temperature（20℃）while higher at 3℃and-20℃;with the extension of storage time,the pollen viability decreased significantly and TTC staining was significantly higher thanin vitro culture method.Two methods for determining had a significant correlation.The suitable condition was at 3℃for‘Xiangling＇,‘Xilin 3＇,‘Xiluo 2＇,‘Chandler＇pollen storage and at-20℃for‘Hartley＇,the storage time was 6-9days.

关 键 词：核桃 花粉生活力 贮藏条件 贮藏方法 

分 类 号：S664.1[农业科学—果树学]