芡实组培一步成苗技术研究  

Study on the One-step Culture of Rapid Propagation Technology in Euryale ferox

作　　者：李彦连[1] 张华丽[2] 赵强[1] 魏晓丽[1] 王艳新[1] 陈杰[1] 张希才[1] 

机构地区：[1]济宁学院生命科学与工程系,山东曲阜273155 [2]济宁医学院药学院,山东日照276826

出　　处：《北方园艺》2015年第16期107-111,共5页Northern Horticulture

基　　金：山东省高等学校科技计划资助项目(J12LE59);济宁学院服务济宁重点资助项目(13FWDF09);济宁市产学研资助项目(2010JNKJ002)

摘　　要：以芡实(Euryale ferox)叶片和茎段为外植体,采用MS培养基,附加2,4-D或NAA以及6-BA或KT,根据正交设计的原理,通过因子分析和聚类分析,研究不同激素配比对芡实一步成苗组培快繁的影响。结果表明:在芡实一步成苗快速繁殖中最佳的激素配比是MS+NAA0.2mg/L+6-BA 2.0mg/L,其余依次是MS+2,4-D 0.5mg/L+6-BA 1.0mg/L、MS+2,4-D0.2mg/L+6-BA 1.0mg/L、MS+2,4-D 1.0mg/L+KT 1.0mg/L、MS+NAA 1.5mg/L+6-BA1.0mg/L。Taking Euryale feroxleaves and stem segments as explants,MS as culture medium,the additional 2,4-D or NAA and 6-BA or KT,according to the principle of orthogonal design,using factor analysis and cluster analysis,the effect of different ratio of hormone on rapid propagation of Gordon Euryale step into seedlings was studied.The results showed that the best hormone matchings was MS＋NAA 0.2 mg/L＋6-BA 2.0 mg/L,others were MS＋2,4-D0.5mg/L＋6-BA 1.0mg/L,MS＋2,4-D 0.2mg/L＋6-BA 1.0mg/L,MS＋2,4-D 1.0mg/L＋KT 1.0mg/L,MS＋NAA 1.5mg/L＋6-BA 1.0mg/L.

关 键 词：芡实 一步成苗 快繁技术 组织培养 

分 类 号：S555.9[农业科学—作物学]