载rhPTH(1-34)纳米缓释纤维促成骨作用的体外研究  

Enhanced Osteogenesis of Polymeric Electrospun Nanofibers Loaded with rhPTH(1-34)in Vitro

作　　者：袁月[1] 刘童斌[1] 孟帅岑 蔡青[1] 张喆[2] 王晶晶[1] 孟维艳[1] 

机构地区：[1]吉林大学口腔医院口腔种植科,吉林长春130021 [2]中国科学院长春应化研究所

出　　处：《口腔医学研究》2015年第8期771-774,共4页Journal of Oral Science Research

基　　金：吉林省发展与改革委员会项目(编号:2013C022-6)

摘　　要：目的:制备载甲状旁腺相关肽(rhPTH(1-34))的电纺丝缓释纤维,研究其缓释效果及对成骨细胞增殖、分化的影响。方法:制备含rhPTH(1-34)电纺丝缓释纤维(PLLA/rhPTH(1-34)),扫描电镜(SEM)观察其形貌。紫外分光光度法测定释放速率。噻唑蓝法(MTT)测定PLLA/rhPTH(1-34)对小鼠前成骨细胞(MC3T3-E1)的增值活性,碱性磷酸酶(ALP)测定其分化。结果:含模型药物的电纺丝缓释纤维体外有效释放21d,PLLA/rhPTH(1-34)缓释纤维促进细胞的增殖作用明显(P<0.05),并能显著促进细胞的分化(P<0.05)。结论:PLLA/rhPTH(1-34)缓释纤维可以有效缓释药物,并能促进MC3T3-E1DE增殖、分化。Objective.. To prepare the electrospun fibers which contained parathyroid related peptide （rhPTH （1-34）） and to study its slow release on the proliferation and differentiation of osteoblasts. Methods.. Electrospinning fibers （PLLA/rhPTH （1-34）） containing slowing released rhPTH （1-34） were prepared. The appearance and the morphology of the fibers were observed with scanning electron microscopy （SEM）. The release kinetics was measured by UV spectrophotometry in vitro. The proliferation activity of MC3T3-E1 cells influenced by PLLA/ rhPTH （1-34） was determined through Thiazole blue method （MTT）. The differentiation of MC3T3-E1 ceils was determined by alkaline phosphatase （ALP）. Results： The sustained-release efficiency day of the spinning fi- bers in vitro was 21. PLLA/rhPTH（1--34） sustained-release fiber could obviously promote the proliferation and differentiation of MC3T3-E1 cells （P〈0.05）. Conclution： PLLA/rhPTH （1-34） loaded fibers can effectively re- lease drugs and promote.the proliferation and differentiation of MC3T3-E1 cells.

关 键 词：电纺丝 甲状旁腺激素 小鼠前成骨细胞 

分 类 号：R783.1[医药卫生—口腔医学]