MonoTrap法捕集白木香愈伤组织挥发性成分及其GC-MS分析  

GC-MS Analysis on the Volatile Constituents Adsorbed with MonoTrap from Callus of Aquilaria sinensis (Lour.) Gilg.

作　　者：张珂[1] 陈华姑 龚胜[1] 孙海燕[1] 王健[1] 

机构地区：[1]海南大学园艺园林学院热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《热带作物学报》2015年第8期1505-1510,共6页Chinese Journal of Tropical Crops

基　　金：海南省中药现代化专项项目(No.ZY201424);海南省应用技术研发与示范推广专项(No.ZDXM2014129)

摘　　要：采用新型材料Mono Trap作为顶空固相萃取的吸附剂,捕集茉莉酸甲酯(Me JA)处理后的白木香愈伤组织挥发性成分,并应用GC-MS分析测定其化学成分及相对含量。结果表明,从Me JA不同处理下的白木香愈伤组织中共鉴定出56种挥发物成分,主要成分为脂肪烃类、芳香族类和倍半萜类等成分;通过正交实验法确定最佳捕集方法为:不破碎愈伤组织材料,于30℃条件下、采用Mono Trap捕集6 h。Mono Trap法结合气质联用具有高灵敏度、强针对性和可操作性强的优点,可以为白木香的开发利用提供理论依据。A novel material Mono Trap was used as the adsorbent of headspace solid phase extraction to absorb the volatile constituents from the Me JA-treated callus of Aquilaria sinensis. Then, the chemical constituents and relative contents of the volatile components were analyzed by GC-MS. The results showed that there were 56 compounds found in the Me JA-treated callus of A. sinensis, in which, the main constituents were aliphatic hydrocarbons, sesquiterpenoids and aromatics. The best absorbing method according to the results of orthogonal experimental was： non-broken callus materials, 30 ℃, absorbing for 6 h. Mono Trap coupled with GC-MS had advantages of high sensitivity, strong pertinence and operability. The study can provide a simpler and more flexible method for the volatile constituents analysis of A. sinensis（Lour.） Gilg.

关 键 词：白木香 愈伤组织 挥发性成分 MonoTrap 气相色谱-质谱 

分 类 号：R284[医药卫生—中药学]