生姜醇提取物抑制人前列腺癌PC-3细胞增殖的体外研究  

Effects of Ethanol Extract from Zingiber on the Proliferation of Human Prostatic Cancer PC-3 Cells in vitro

作　　者：蒋茂林[1] 王毅[2] 曹正国[1] 张朝胜[1] 黎建欣[1] 肖恒军[3] 彭潋[1] 蒋鸿涛[2] 

机构地区：[1]粤北人民医院医院泌尿外科,广东韶关512026 [2]南华大学附属第二医院肾移植泌尿中心,湖南衡阳421001 [3]中山大学附属第三医院泌尿外科,广东广州510630

出　　处：《肿瘤药学》2015年第4期275-278,共4页Anti-Tumor Pharmacy

基　　金：韶关市医药卫生科研计划项目(Y13153)

摘　　要：目的观察生姜醇提取物(extract of zingiber ethanol,EZE)对人前列腺癌PC-3细胞体外增殖的影响,并探讨其可能的调控机制。方法运用MTT法检测不同浓度EZE对PC-3细胞体外增殖的影响;Westernblot检测不同浓度EZE作用于PC-3细胞一定时间后其P53、Bcl-2蛋白的表达情况。结果 MTT法结果提示,随着EZE浓度的增加与作用时间的延长,PC-3细胞DE增殖抑制率均增加,与对照组比较均有显著差异(P<0.05);Western-blot结果显示EZE作用PC-3细胞后可上调P53、下调Bcl-2蛋白的表达,并呈一定的量效关系。结论 EZE能够抑制PC-3细胞体外增殖,其机制可能与其上调P53、下调Bcl-2蛋白的表达有关。Objective To investigate the effects of ethanol extracts from Zingiber（EEZ） on the proliferation of human prostatic cancer PC-3 cells（PC-3） and its possible mechanisms. Methods MTT was used to analyze the effects of different doses of ethanol extract of Zingiber on the proliferation of human Prostatic cancer PC-3 cells. Western-blot was applied to detect the expressions of bcl-2 and p53 protein in PC-3 cells after treated by EEZ. Results MTT assay showed that the inhibition rate of cell proliferation all increased along with the increase of concentration and interaction time of EEZ. But it was significantly higher in the experimental group than in the control group（P〈0.05）. Western-blot assay showed that P53 protein expression increased while Bcl-2 protein expression reduced within a certain range of treating time and dose in PC-3 cells treated by EEZ. Conclusion Ethanol extract of Zingiber could inhibit the proliferation of PC-3 cells in vitro within a certain range of treating time and dose. The increase of P53 protein expression and decrease of Bcl-2 protein expression might be involved.

关 键 词：生姜醇提取物 前列腺癌 增殖 PC-3细胞 

分 类 号：R737.25[医药卫生—肿瘤]