香菇中污染真菌的分离与鉴定  

Isolation and identification of fungus contaminated in Lentinula edodes

作　　者：冯智红 严培兰 王建华[2] 韩铮[2] 邢增涛[1,2] 武爱波[1,2] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [3]上海瑞丰农业科技有限公司,上海201106

出　　处：《上海农业学报》2015年第4期50-53,共4页Acta Agriculturae Shanghai

基　　金：国家自然科学基金项目(31301493)

摘　　要：从上海及周边地区的香菇子实体和培养料中分离污染真菌,并进行形态学观察和分子生物学鉴定。提取菌株基因组DNA,利用真菌通用引物ITS1和ITS4扩增菌株rDNA ITS区序列,测序后在数据库中进行比对分析,并利用软件:MEGA5构建菌株分子系统发育树。结果表明:香菇栽培到储藏过程中主要污染真菌有木霉属(Trichoderma)、曲霉属(Aspergillus)、附球菌属(Epicoccum)、镰孢属(Fusarium)、青霉属(Penicillium)和节菱孢属(Arthrinium)等。Symbiotic fungus from fruit body and substrate of Lentinula edodes in and around Shanghai were separated for morphological observation and molecular identification. By determining the sequence of a ribosomal DNA internal transcribed spacer （ITS） , and comparing with corresponding sequences deposited in NCBI based on the closest match of BLAST analysis, the strains molecular phylogenetic tree was built using MEGAS. The re- sults showed that the main fungus contaminated during the process of cultivation to storage were Arthrinium, Epic- occum , Fusarium , Penicillium , Trichoderma and Aspergillus.

关 键 词：香菇 污染真菌 形态观察 分子鉴定 

分 类 号：S852.653[农业科学—基础兽医学]