ADSCs抑制辐射诱生胸腺瘤的作用分析  

Inhibition effect of ADSCs on thymomas induced by irradiation

作　　者：熊志[1,2] 孔志华[1,2] 朱俊[1,2] 袁玉林[2] 王国祥[2] 夏干清 

机构地区：[1]湖北省咸宁市中心医院同济咸宁医院,湖北咸宁437000 [2]武汉大学基础医院解剖教研室,湖北武汉430072

出　　处：《海南医学院学报》2015年第8期1009-1013,共5页Journal of Hainan Medical University

基　　金：湖北省自然科学基金项目(301132174)~~

摘　　要：目的:探讨脂肪来源干细胞(ADSCs)对辐射诱导形成胸腺瘤的影响。方法:清洁级C57BL/6小鼠160只随机分为4组:假照射组、照射组、照射+ADSCs组、假照射+ADSCs组,各40只,末次照射后第1、3、7、14天后分别处死4组小鼠,荧光显微镜下观察ADSCs在胸腺瘤组织中的定位;末次照射第1、7、14、21、28天及6个月后分别处死4组小鼠,石蜡包埋HE染色观察胸腺组织的病理变化,并行免疫组化分析。结果:照射组小鼠胸腺瘤的发生率(75%,12/16)明显高于ADSCs注射组(20%,3/15)(P<0.05)。在X线照射后第7天和6个月后,照射组和照射+ADSCs组胸腺组织中CD31染色阳性的血管管状结构及增殖细胞核抗原(PCNA)阳性细胞数较假照射组和假照射+ADSCs组明显增多,并且照射+ADSCs组小鼠胸腺组织的PCNA阳性细胞数相对于照射组小鼠胸腺组织较低(P<0.05)。结论:本实验成功建立辐射诱发的小鼠胸腺瘤模型。ADSCs具有保护胸腺辐射损伤,抑制辐射诱发胸腺瘤的作用。Objective.. To evaluate that the effect of adipose-derived stem cells （ADSCs） on thymomas induced by irra- diation. Methods.. A total of 160 cleaning degree C57BL/6 mice were divided into four groups randomly., control group of 40 mice with non-irradiation; irradiation group of 40 mice with irradiation; irradiation＋ADSCs group with 40 mice, thymoma model mice injected with 0.5 mL ADSCs via tail vein at one day after last irradiation; non-irradiation＋ ADSCs group of 40 mice with the same ADSCs injection as irradiation＋ADSCs group. All mice were sacrificed on the 1st, 3rd, 7th and 14th day after last irradiation, localization of ADSCs in thymoma tissue was detected using fluorescence microscope. Four groups mice were sacrificed on the 1st, 3rd, 7th, 14th day and the 6th month after last irradiation,pathological changes of thymus gland tissue were observed by HE staining and immunohistochemistry assay. Results： The thymoma incidence of irradiation＋ADSCs group was significantly lower in control group. The expression of CD31 and PCNA in irradiation group and irradiation＋ ADSCs group mice was significantly higher than that of control group, and the expression of PCNA in irradiation＋ ADSCs group mice was significantly lower than that of radiation group mice. Oonclusios： ADSCs can reduce the degree of irradiation damage of thymus tissue and inhibit the growth of thymoma induced by irradiation.

关 键 词：脂肪来源干细胞 辐射 胸腺瘤 

分 类 号：R736.3[医药卫生—肿瘤]