USP22基因在氟尿嘧啶对肝癌细胞毒性作用中的影响  

Influence of USP22 Gene in Cytotoxic Effect of Fluorouracil on Hepatocarcinoma Cells

作　　者：许涛[1] 张磊[2] 杨涛[1] 曹好好[1] 熊俊[2] 

机构地区：[1]华中科技大学同济医学院附属普爱医院重症医学科,湖北武汉430033 [2]华中科技大学同济医学院附属协和医院肝胆外科,湖北武汉430022

出　　处：《武汉大学学报（医学版）》2015年第5期677-680,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金项目(编号:2011CDB103)

摘　　要：目的:探讨USP22基因在氟尿嘧啶对肝癌细胞毒性作用中的影响。方法:用Western blotting检测肝癌细胞HepG2和BEL7402中USP22基因的表达状态,构建并对肝癌细胞转染USP22基因的siRNA(siRNAUSP22),然后以氟尿嘧啶处理24h后,MTT检测法观察在USP22基因沉默前后,氟尿嘧啶对肝癌细胞活性的影响。结果:USP22基因在肝癌细胞中显著高表达。siRNA-USP22转染后显著抑制USP22基因的表达,同时可明显增强氟尿嘧啶对HepG2和BEL7402细胞的毒性作用,降低其细胞活性[(53.2±6.8)%,P<0.05;(49.8±7.3)%,P<0.05]结论:下调USP22基因可提高肝癌细胞对氟尿嘧啶的敏感性,提示USP22高表达可能是造成肝癌化疗抵抗的原因之一。Objective： To investigate the influence of USP22 gene in cytotoxic effect of fluorouracil on hepatocarcinoma cells. Methods： Expression status of USP22 gene was detected by Western blot- ting in HepG2 and BELT402 hepatocarcinoma cell lines, siRNA of USP22 （siRNA-USP22） was constructed and transfected in hepatocarcinoma cells for 24 h. The influence of fluorouracil to hepatocarcinoma cellular activity was assessed by MTT assay before and after USP22 silence. Results： USP22 was significantly upregulated in hepatocarcinoma cells. Transfection of siRNA- USP22 obviously inhibited expression of USP22 in hepatocarcinoma cells and enhanced the cyto- toxic effect of fluorouracil on HepG2 and BEL7402 cells. Their cellular activities decreased to （3.2±6. 8）% and （49. 8± 7. 3）V0 respectively （P〈0. 05）. Conclusion： Downregulation of USP22 may enhance the sensitivity of hepatocarcinoma cells to fluorouracil, suggesting that increased expression of USP22 may be one of the reasons for hepatocarcinoma chemotherapy resistance.

关 键 词：USP22 肝癌细胞 氟尿嘧啶 化疗 

分 类 号：R735.7[医药卫生—肿瘤]