Livin、Caspase-3蛋白在不同胃病变组织中表达及其临床意义  

EXPRESSION OF LIVIN AND CASPASE-3 PROTEIN IN DIFFERENT STOMACH TISSUES AND ITS CLINICAL SIGNIFICANCE

作　　者：杨彬[1] 赵瑛[1] 王丽华[1] 曹洁莉 陈玉梅[1] 

机构地区：[1]河北省衡水市哈励逊国际和平医院,053000

出　　处：《中国煤炭工业医学杂志》2015年第9期1469-1473,共5页Chinese Journal of Coal Industry Medicine

基　　金：河北省衡水市卫生局(编号:14009A)

摘　　要：目的探讨Livin、Caspase-3蛋白在不同胃病变组织中的表达,以及其二者之间的关系。方法选择就诊于哈里逊国际和平医院2013年3月—2014年3月确诊为慢性非萎缩性胃炎(chronic non atrophic gastritis)患者20例,慢性萎缩性胃炎(chronic atrophic gastritis,CAG)非肠化患者30例,CAG伴肠化患者30例,胃癌患者30例,其中低分化组10例,中分化组10例,高分化组10例为研究对象,Livin、Caspase-3蛋白应用免疫组织化学实验(IHC)检测。结果 Livin蛋白在慢性非萎缩性胃炎、CAG非肠化、CAG伴肠化、胃癌组织中阳性率分别为0、40.00%、43.33%、60.00%,差异均有统计学意义(P<0.001),胃癌组织低、中、高分化三组之间差异无统计学意义(P>0.05);Caspase-3蛋白在慢性非萎缩性胃炎、CAG非肠化、CAG伴肠化、胃癌组织中阳性率分别为90.00%、53.33%、46.67%、30.00%,差异均有统计学意义(P<0.001);在胃癌组织低、中、高分化三组之间差异无统计学意义(P>0.05);Livin、Caspase-3蛋白在不同胃病变组织中的表达呈负相关(r=-0.505,P<0.05);二者的表达与性别、年龄无统计学意义(P>0.05)。结论 Livin可能与Caspase-3蛋白共同作用促进胃癌的发生、发展,Livin可做为独立的预后因素,并且有望成为早期胃癌的诊断标志物。Objective To investigate the expression of Livin, Caspase- 3 protein in different gastric lesion tissues, and the relationship between Livin and Caspase- 3 protein. Methods From March 2013 to March 2014, some patients diagnosed in author＇s hospital were selected, included 20 cases of patients with chronic non atrophic gastritis, 30 cases of chronic atrophic gastritis without intestinal metaplasia, 30 cases of CAG with intestinal metaplasia, and 30 cases of gastric carcinoma （ low differentiation group, middle - differen- tion group, high differentiation group, 10 cases in each group）. Expression of Livin, Caspase- 3 protein was detected by immunohistochemieal assay （IHC）. Results Positive expression of Livin protein in chronic non atrophic gastritis, CAG without intestinal metaplasia, CAG with intestinal metaplasia, gastric carcino- ma were 0%, 40%, 43.33% and 60%, respectively, the difference was statistically significant （P〈0. 001 ）, but there were no statistical difference on expression of the proteins in gastric cancer with low, mid- dle, high differentiation （P〉0.05）. Positive expression of Caspase - 3 protein in chronic non atrophic gas- tritis, CAG without intestinal metaplasia, CAG with intestinal metapiasia, gastric carcinoma were 90%, 53.33%, 46.67% and 30%, respectively, the difference was statistically significant （ P〈0. 001 ）, but in gastric cancer with low, middle, high differentiation were no statistically significant （P〉0.05）. It was negatively related to expression of Livin, Caspase- 3 protein in different gastric lesion tissues （r = - 0. 505, P〈0.05） ; their expression were no relationship with gender, age of patients （P〉0.05）. Conclusion Livin promotes the occurrence and development of gastric cancer through joint action with caspase - 3 protein, it can be used as an independent prognostic factor, can be used as damage indicators response in gastric cancer markers.

关 键 词：LIVIN CASPASE-3 胃癌 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]