长链非编码RNA MALAT1调控Rac1b表达与结直肠癌侵袭和转移的关系  

Long noncoding RNA MALAT1 regulates Rac1b expression and is associated with colorectal cancer metastasis

作　　者：杨孜欢[1] 冯杏芝 方乐堃[1] 黄丹丹[1] 汪建平[1] 

机构地区：[1]中山大学附属第六医院,广东省结直肠盆底疾病研究重点实验室,广东省胃肠病学研究所,广东广州510655

出　　处：《中国病理生理杂志》2015年第8期1417-1421,共5页Chinese Journal of Pathophysiology

基　　金：教育部留学回国人员科研启动项目(教外司留[2013]693号);广东省医学科研基金资助项目(粤卫[2013]33号;No.B2013161)

摘　　要：目的:探讨MALAT1调控Rac1b的表达与结直肠癌侵袭和转移的关系。方法:结直肠癌患者组织样本手术切除后30 min内于-80℃保存;实时荧光定量PCR检测肿瘤组织和配对正常组织中MALAT1和Rac1b mRNA的表达;RNA干扰沉默结直肠癌细胞MALAT1表达,通过Western blot检测Rac1b和上皮间质化标志物的表达,Ed U实验检测其对细胞增殖的影响;Transwell小室实验检测MALAT1低表达后对细胞迁移和侵袭的影响。结果:MALAT1在结直肠癌中低表达;干扰MALAT1表达后,Rac1b的表达升高,细胞增殖加快,且上皮间质化标志物E-cadherin和β-catenin表达升高。干扰MALAT1的表达可明显促进结直肠癌侵袭和细胞的迁移和侵袭。结论:MALAT1低表达与结直肠癌侵袭和转移密切相关,并且这一相关性是通过调控Rac1b的表达实现的。AIM： To investigate the role of MALAT1 in colorectal cancer metastasis. METHODS： The mRNA expression levels of MALAT1 and Rac1 b in the tumor and adjacent normal tissues were examined by real-time PCR.MALAT1 was knocked down by siRNA in colorectal cancer cell lines. The expression of Rac1 b and the epithelial-mesenchymal transition markers was examined by Western blot. Cell proliferation was determined by Ed U analysis. The effects of MALAT1 on the cell migration and invasion were examined by Transwell assay. RESULTS： The expression of MALAT1 was down-regulated in colorectal cancer. Down-regulation of MALAT1 induced Rac1 b overexpression,which in turn increased the expression levels of E-cadherin and β-catenin. Furthermore,down-regulation of MALAT1 promoted the cell proliferation,invasion and migration. CONCLUSION： MALAT1 is associated with metastasis of colorectal cancer through regulating the expression of Rac1 b and the downstream factors.

关 键 词：长链非编码RNA 肺腺癌转移相关转录本1 结直肠癌 肿瘤转移 Rac1b 

分 类 号：R574[医药卫生—消化系统]