健脾补肾方对辐射损伤小鼠β-catenin、TCF及PPARγ蛋白表达的影响  

Effect of Jianpi Bushen Prescription on expressions of β-catenin,TCF and PPARγ proteins in radiation-damaged mice

作　　者：何东初[1] 肖静静[1] 张勇[1] 林慧[1] 丁晓娟[1] 涂颖[1] 

机构地区：[1]广州军区武汉总医院,湖北武汉430070

出　　处：《现代中西医结合杂志》2015年第26期2851-2854,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81273905)

摘　　要：目的研究健脾补肾方对辐射损伤模型小鼠β-链蛋白(β-catenin)、T细胞因子(TCF)及过氧化酶体增殖物激活受体γ(PPARγ)蛋白表达的影响,探讨其对造血功能的调控机制。方法采用6.0 Gy X射线照射建立辐射损伤模型小鼠,然后将造模成功小鼠随机分为模型组、健脾补肾方小剂量组(100%浓度)、健脾补肾方大剂量组(200%浓度)、阿胶浆组,每组12只。另随机选取12只正常小鼠作为正常组。正常组、模型组给予生理盐水0.2m L/10 g灌胃,2次/d,其余3组分别用健脾补肾方小剂量、大剂量和阿胶浆混悬液0.2 m L/10g灌胃,2次/d。9 d后处死小鼠,取血检测外周血白细胞计数,收集骨髓检测β-catenin、TCF及PPARγ蛋白表达情况,制作骨髓病理切片观察骨髓病理改变情况,测定脂肪空泡面积。结果模型组外周血白细胞计数及β-catenin、TCF蛋白表达量明显低于正常组(P均<0.05),PPARγ蛋白表达量及骨髓脂肪空泡面积均明显高于正常组(P均<0.05);健脾补肾方小剂量组、健脾补肾方大剂量组、阿胶浆组外周血白细胞计数及β-catenin、TCF蛋白表达量均明显高于模型组(P均<0.05),PPARγ蛋白表达量及骨髓脂肪空泡面积均明显低于模型组(P均<0.05),尤以小剂量组明显。结论健脾补肾方可通过增强β-catenin/TCF通路的转导,抑制下游靶基因PPARγ的表达,从而抑制骨髓脂肪化,促进骨髓造血功能的重建。Objective It is to investigated the effect of Jianpi Bushen Prescription（ JBP） on the expression of β- catenin,TCF and PPARγ proteins in radiation-damaged mice and explore the regulating mechanism on hematopoietic function. Methods The mouse models of radiation damage were established by 6. 0 Gy X- rays radiation exposure for 9 days. Then the successful models of mice were randomly divided into model group,low-dose（ 100%） JBP group,high-dose（ 200%） JBP group,compound e- jiao slurry（ EJS） group,each group had 12 mice. Another 12 normal mice were selected as normal group. The normal group,model group was given normal saline 0. 2 m L /10 g by gavage twice per day,the other three groups were respectively given low-dose JBP,high-dose JBP,EJS 0. 2 m L /10 g by gavage twice per day. After 9 days,the mice were killed to get their blood to determine peripheral white blood cells,the expressions of β- catenin,TCF and PPARγ proteins were detected in bone marrow,pathological section of bone narrow was made to observe the changes of pathology,and the proportion of adipocyte area were measured. Results After irradiation,the number of white blood cells decreased obviously,the protein expression of β- catenin,TCF proteins were significantly downregulated,the protein expression of PPARγ protein was significantly upregulated and the percentage of adipocyte area was increased,in the model group compared to the control group（ P〈0. 05）. The low dose JBP,high dose JBP,and e- jiao slurry treatments significantly upregulated the protein expression of β- catenin,TCF proteins and downregulated the protein expression of PPARγ protein,the percentage of adipocyte area was reduced compared to the model group（ P〈0. 05）,with the low dose JBP producing the best results. Conclusion JBP plays a protective role on bone narrow hematopoietic function through the activation of the β- catenin / TCF signaling pathway,and inhibited adipogenesis by redcucing the expression of PPARγ.

关 键 词：健脾补肾方 辐射损伤 Β-CATENIN TCF PPARΓ 动物模型 

分 类 号：R-332[医药卫生]