自制混合血浆在血浆纠正试验中的临床应用初探  

Preliminary investigation on clinical application of homemade mixed plasma in plasma correcting test

作　　者：龚娅[1] 何宗忠[1] 王晓冬[1] 史秋霞[1] 林林东[1] 

机构地区：[1]广州军区广州总医院157分院检验科,广州510510

出　　处：《检验医学与临床》2015年第16期2357-2358,2361,共3页Laboratory Medicine and Clinic

摘　　要：目的通过比较不同温度保存的自制混合血浆与正常混合血浆对血浆纠正试验的影响,探讨自制混合血浆在血浆纠正试验中的临床应用价值。方法收集凝血酶原时间(PT)≥18.0s和(或)活化部分凝血活酶时间(APTT)≥52.0s的血浆标本35份,分别与正常混合血浆(A组)、-80℃(B组)和-20℃(C组)保存的自制混合血浆按体积1∶1混合后做血浆纠正试验,即混合后立即测定PT和(或)APTT,37℃孵育1h后再次检测。计算35份标本可被正常混合血浆纠正的比例及3组的纠正率。结果 88.57%(31/35)可被正常混合血浆立即纠正,4例未被纠正,其中1例孵育后比孵育前测定时间更加延长,占2.86%;3例孵育前后测定结果不变,占8.57%。在PT延长的血浆纠正试验中,各组之间纠正率差异无统计学意义(P>0.05)。在APTT延长的纠正试验中,孵育前A组、B组和C组的纠正率分别为(32.93±16.17)%、(27.56±23.48)%和(13.70±12.87)%,A组和B组之间纠正率差异无统计学意义(P>0.05),其余各组之间纠正率差异有统计学意义(P<0.05)。结论 -80℃保存的自制混合血浆可代替商品化的正常混合血浆进行血浆纠正试验。Objective To explore the clinical application value of homemade plasma in the plasma correct test by comparing the influence of normal mixture plasma and homemade plasma storage at different temperatures. Meth- ods 35 plasma samples of prothrombin time（PT）≥18.0 s and/or activated partial thromboplastin time （APTT）≥ 52.0 s were collected and mixed with the normal mixture plasma（group A）, homemade plasma stored at --80 ℃ （group B） and --20 ℃ （group C） with the proportion of 1 ： 1 respectively,then PT and APTT were tested immedi- ately,and retested after 37 ℃ incubating for 1 h,finally the correcting proportion in 35 samples and the correct ratio of these three groups were calculated. Results In the 35 cases of PT and/or APTT prolongation,31 cases （88. 570/00） could be corrected by the normal mixture plasma and 4 cases were not corrected, among them, the detection time after incubation in 1 case（2.86 %） was prolonged than that before incubation,and the detection results before and after in- cubation in 3 cases were unchanged,accounting for 8.57%. In the PT prolonged correction test,there was no statisti eal difference in the correct ratio among 3 groups （P〉0.05）. In the APTT prolonged correction test, the correcting ratios before incubation in the group A,B and C were （32.93± 16.17） % ,（27.56±23.48）% and （13.70± 12.87） respectively,there was no statistical difference in the correct ratio between the group A and B （P〉0.05）, which in the other groups had statistical diffetenee（P〈0.05）. Conclusion The homemade plasma stored at -80℃ can re- place the commercialization normal mixture plasma for conducting the plasma correcting test.

关 键 词：正常混合血浆 血浆纠正试验 凝血酶原时间 活化部分凝血活酶时间 

分 类 号：R737.9[医药卫生—肿瘤]