盆炎灵颗粒HPLC指纹图谱研究  

HPLC fingerprints of Penyanling granules

作　　者：肖作奇 潘涛[1] 欧阳波[1] 黄懿[1] 田霞[1] 文晓柯 

机构地区：[1]湖南省妇幼保健院,长沙410008

出　　处：《中国新药杂志》2015年第16期1897-1901,共5页Chinese Journal of New Drugs

基　　金：湖南省中医药管理局科研项目(201402);长沙市科技局科技项目(K12ZD010-33);湖南省科技厅科技项目(2013SK3212)

摘　　要：目的:采用HPLC建立盆炎灵颗粒指纹图谱分析方法。方法:色谱柱为Hypersil ODS2柱(250 mm×4.6 mm,5μm);流速:1.0 m L·min-1;流动相A:乙腈,流动相B:0.1%磷酸水溶液,梯度洗脱,柱温:25℃,检测波长为280 nm。结果:建立了盆炎灵颗粒的HPLC指纹图谱,标示出36个共有色谱峰,颗粒与各药材具有很好的相关性,同时对其中11个色谱峰进行归属。结论:建立的方法灵敏、准确、简单、分离效果好,可作为盆炎灵颗粒质量评价的方法。Objective： To establish an HPLC fingerprint method for Penyanling granules. Methods： A Hypersil ODS2 column was used, and the flow rate was 1.0 mL· min^ -1. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid solution with gradient elution at 25 ℃. The detection wavelength was set at 280 nm. Results： The common modes for the HPLC fingerprints were set up by ensuring 36 common peaks in the chromatograms. The relativity was distinct between Penyanling granules and the relevant herbs. Eleven chemical constituents were analyzed qualitatively. Conclusion： This method is sensitive, accurate and simple, and can be used for the quality control of Penyanling granules.

关 键 词：盆炎灵颗粒 高效液相色谱 指纹图谱 相关性 

分 类 号：R914.1[医药卫生—药物化学]