二维高通量色谱分离制备海参来源真菌Epicoccum sp.的化学成分  

Separation and preparation of chemical components from sea cucumber-derived fungus Epicoccum sp. by two-dimensional high-throughput chromatography

作　　者：齐君 夏雪奎[1] 贾爱荣[2] 刘新[2] 张绵松[1] 刘昌衡[2] 

机构地区：[1]山东省科学院中日友好生物技术研究中心山东省应用微生物重点实验室,山东济南250014 [2]山东省科学院生物研究所,山东济南250014

出　　处：《山东科学》2015年第4期14-18,24,共6页Shandong Science

基　　金：山东省科学院科学技术发展计划(2014QN018);国家自然科学基金(81202452);中韩国际合作交流项目(81411140251)

摘　　要：建立了二维高通量色谱分离、纯化海参来源真菌Epicoccum sp.中的海松烷二萜化合物的新方法。以甲醇-水为溶剂体系梯度洗脱,在流速为40 m L/min、主分离检测波长为254 nm,二级分离检测波长为220 nm的条件下进行分离,从1.5 g海参来源真菌Epicoccum sp.乙酸乙酯提取物中分离制备得到海松烷二萜化合物,通过核磁共振氢谱和质谱鉴定化合物的结构。该方法简便、快捷且减少样品的不可逆吸附,为海松烷二萜化合物的制备型分离提供了新手段。We established a new method for separation and preparation of pimarane diterpenes from sea cucumber- derived fungus Epicoccum sp. by two-dimensional high-throughput chromatography. It was performed with MeQH-H^O as solvent, flow rate of 40 mL/min, main separation detection wavelength of 254 nm and second separation detection wavelength of 220 nm. Pimarane diterpenes were isolated from ]. 5 g ethyl acetate extract fromsea cucumber-derived fungus Epicoccum sp. Their structureswere identified by spectroscopic data （ ~ H NMR and MS）. The method is simple and rapid, reduces irreversible sample adsorption, and provides a new approach for separation and preparation of pimarane diterpenes.

关 键 词：二维高通量分离制备色谱 海参来源真菌 海松烷二萜化合物 

分 类 号：R284.2[医药卫生—中药学]