牛磺酸对海兰褐雏鸡抗氧化及免疫功能的影响  

Effect of taurine on antioxidant and immunity of Hailan-brown chicks

作　　者：赵振升[1] 位治国[1] 杨帆[1] 王国永[1] 郝雪琴[1] 孔涛[1] 潘耀谦[2] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]河南科技学院动物疫病和残留物防控河南省高校工程技术研究中心,河南新乡453003

出　　处：《中国兽医科学》2015年第8期867-872,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(3110086)

摘　　要：选取10日龄海兰褐雏鸡240只,随机将其分为8组。在牛磺酸处理试验中Ⅰ、Ⅱ、Ⅲ、Ⅳ为正常给药组,分别在日粮中添加牛磺酸0、0.5、1.0、2.0g/kg。在地塞米松(Dex)牛磺酸处理试验中A、B、C、D组为地塞米松处理组(混饮,25mg/L),并分别添加牛磺酸0、0.5、1.0、2.0g/kg。在给药后第15天和第30天测定各项抗氧化指标。结果显示,在牛磺酸处理试验中,与对照组(Ⅰ)相比,超氧化物歧化酶(SOD)活性在第15天Ⅲ和Ⅳ组显著升高(P<0.05);在第30天Ⅱ组显著升高(P<0.05),Ⅲ和Ⅳ组极显著升高(P<0.01)。过氧化氢酶(CAT)活性在第15天Ⅲ组显著升高(P<0.05),Ⅱ和Ⅳ组变化不显著(P>0.05);在第30天Ⅲ组极显著升高(P<0.01),Ⅳ组显著升高(P<0.05),Ⅱ组变化不显著(P>0.05)。丙二醛(MDA)含量,在第15天和第30天Ⅱ组极显著降低(P<0.01),Ⅲ和Ⅳ组显著降低(P<0.05)。胸腺指数、脾指数和法氏囊指数,在第15天Ⅱ、Ⅲ和Ⅳ组均无显著变化(P>0.05);在第30天Ⅲ组胸腺指数显著增大(P<0.05),Ⅱ、Ⅲ和Ⅳ组脾指数均显著增大(P<0.05),Ⅲ和Ⅳ组法氏囊指数显著增大(P<0.05)。抗体效价在第15天和第30天Ⅱ、Ⅲ和Ⅳ组均显著提高(P<0.05)。在Dex牛磺酸处理试验中,血清SOD活性在第30天C和D组显著提高(P<0.05)。血清CAT活性在第30天B组极显著降低(P<0.01),C组极显著升高(P<0.01),D组显著降低(P<0.05)。血清MDA含量在第15天C组显著降低(P<0.05),在第30天B组显著降低(P<0.05)。胸腺指数、脾指数、法氏囊指数和抗体效价各组均无显著变化(P>0.05)。上述结果提示,牛磺酸可提高正常海兰褐雏鸡的抗氧化作用和免疫功能,对经过Dex处理的海兰褐雏鸡,牛磺酸也表现出抗氧化作用,但对免疫功能影响尚不能确定。Two hundred and forty 10-day-old chicks were randomly divided into 8 groups. In the experiment of taurine,feeds in Group Ⅰ , Ⅱ , Ⅲand Ⅳ were added independently with 0,0.5,1.0 and 2.0 g/kg taurine,respectively. In the experiment of dexamethasone（Dex） and taurine,Group A,B,C and D had been treated by drinking Dex（25 mg/L）,then raised feeds supplemented with 0,0.5,1.0 and 2.0 g/kg taurine, respectively. The every index was tested on the 15th and 30th days post-treatment. In result,in the experiment of taurine,the level of serum SOD was increased significantly in GroupⅢ and Ⅳ （P〈0.05） on the 15th day,significantly increased in Group Ⅱ （P〈0.05） and increased very significantly in GroupⅢ and Ⅳ （P〈0.01） on the 30th day. The level of serum CAT was increased significantly in Group Ⅲ （P〈0.05） but no significant changes were observed in Group Ⅱ and Ⅳ（P〉0.05） on the 15th day,while on the 30th day very significant changes（P〈0.01）, significant changes（P〈0.05） and no significant ehanges（P〉0. 05） were observed in GroupⅢ , Ⅳand Ⅱ ,respectively. The level of serum MDA decreased very significantly in Group Ⅱ （P〈0.01） ,and significantly in Group m and Ⅳ （P〈0.05） on the 15th and 30th days. The indexes of thymus,spleen and bursa of Fabricius have no significant changes in Group Ⅱ ,Ⅲ and Ⅳ（P〉0.05） on the 15th day. All the thymus index in GroupⅢ ,the spleen index in Group Ⅱ , Ⅲ and Ⅳ, the bursa of Fabricius index in GroupⅢ and Ⅳ were aggrandized significantly（P〈0.05） on the 30th day. The titers of antibody were enhanced significantly in Group Ⅱ , Ⅲ and Ⅳ （P〈0.05） on both the 15th and 30th day. In the Dex-taurine experiment,the level of serum SOD increased significantly in Group C and D（P〈0.05） on the 30th day. The level of serum CAT decreased very significantly（P〈0.01） in Group B,while was aggrandized very significantly in Group C （P〈0. 01） ,and decreased significantly（P〈

关 键 词：牛磺酸 地塞米松 抗氧化 免疫 

分 类 号：S852.3[农业科学—基础兽医学]