EB病毒miR-BART4~＊和miR-BART18-3p在鼻咽癌中的表达及意义  

Expression of EB virus miR-BART4~* and miR-BART18-3p in nasopharyngeal carcinoma and its significance

作　　者：李璐[1] 王建红[1] 黄辉[2] 范才文[1] 肖胜军[1] 熊伟明[2] 雷迅[2] 

机构地区：[1]桂林医学院生物化学与分子生物学教研室,桂林541004 [2]桂林医学院附属医院耳鼻咽喉头颈外科,桂林541001

出　　处：《安徽医科大学学报》2015年第9期1267-1270,共4页Acta Universitatis Medicinalis Anhui

基　　金：广西自然科学基金项目(编号:2011GXNSFD018034);广西教育厅科研项目(编号:2013YB168);广西医学科学实验中心开放基金专项项目(编号:KFJJ2010-51);广西科学研究与技术开发计划项目(编号:桂科攻0592007-1J)

摘　　要：目的分析EB病毒(EBV)miRNAs在鼻咽癌组织中的表达,探索EBV诱发鼻咽癌的分子机制,寻找鼻咽癌防治和诊断的生物分子标志物。方法采用实时荧光定量PCR分析EBV miRNAs ebv-miR-BART4*和ebv-miR-BART18-3p在36例鼻咽低分化鳞癌组织和32例慢性鼻咽黏膜炎症组织中的表达水平。结果ebv-miR-BART4*和ebv-miRBART18-3p的表达水平(Ct值)在鼻咽癌组织中分别为(26.97±2.67)和(27.02±2.32),在鼻咽黏膜炎症组织中的表达水平分别为(38.49±3.05)和(39.39±2.16),两种EBV miRNAs在鼻咽癌组织中的表达明显高于鼻咽黏膜炎症组织,差异有统计学意义(P<0.01)。结论 EBV可能通过ebv-miR-BART4*和ebv-miR-BART18-3p高表达诱发鼻咽癌,它们是鼻咽癌诊断和防治的潜在生物分子标志物。Objective To analyze expression of EB virus （EBV） miRNAs in nasopharyngeal carcinoma（NPC） tis- sue, explore molecular mechanism of EBV induced NPC and biomarker for prevention and diagnosis of NPC. Meth- ods 36 cases of nasopharyngeal poorly differentiated squamous cell carcinoma tissues and 32 cases of chronic na- sopharyngeal mucositis tissues were collected. Expressions of EBV miRNAs ebv-miR-BART4 ＊ and ebv-miR- BART18-3p were tested in these tissues by quantitative real-time PCR; the expression level of EBV miRNAs was analyzed in both tissue groups. Results The expressions（Ct） of ebv-miR-BART4＊ and ebv-miR-BART18-3p were （26.97 ±2.67） and （27.02 ±2. 32） in NPC tissue, （38.49 ±3.05） and （39.39±2. 16）in nasopharyngeal mu- cosa inflammation tissue, the expressions of two ebv-miRNAs were significantly higher in NPC tissue than that in nasopharyngeal mucosa inflammation tissue （ P 〈 0. 01 ）. Conclusion NPC may be induced by EBV through the high expression of ebv-miR-BART4 ＊ and ebv-miR-BART18-3p. They are potential biological molecular markers for the diagnosis and treatment of NPC.

关 键 词：鼻咽癌 生物分子标志物 EB病毒微小RNA 

分 类 号：R739.63[医药卫生—肿瘤]