胶质瘤干祖细胞体外诱导恶性转化的树突状细胞株SU3-ihDCTC的建立及其特征分析  

Establishment and characteristics of a malignant transformed dendritic cell line SU3-ihDCTC in vitro in- duced by glioma stem progenitor cells

作　　者：王德林[1] 代兴亮[1] 费喜峰[3] 王海洋[1] 陈金生[1] 王麒龙 芮琴[2] 王之敏[3] 王爱东[2] 董军[1] 兰青[1] 黄强[1] 

机构地区：[1]苏州大学附属第二医院神经外科,215004 [2]苏州大学附属第二医院实验中心,215004 [3]苏州大学九龙医院神经外科

出　　处：《中华微生物学和免疫学杂志》2015年第7期517-522,共6页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金（81172400,81302196,81472739）

摘　　要：目的探讨胶质瘤干祖细胞体外诱导恶性转化的树突状细胞株的建立及其特征分析。方法取表达绿色荧光蛋白（EGFP）的小鼠骨髓腔冲洗细胞，用树突状细胞诱导培养方法，与表达红色荧光蛋白（RFP）的人胶质瘤干祖细胞SU3共培养，从单克隆增殖能力强的EGFP（＋）永生化细胞中随机选取1株检测相关分子标志物，行染色体分析和致瘤试验。结果被鉴定分析的1株永生化EGFP（＋）细胞形态为树突状，Dc标志蛋白CD11c、CD80、信号调节蛋白α（SIRP-α）均高表达，此外表达宿主鼠源性β-肌动蛋白和EGFP；且兼具高增殖、侵袭和移植致癌的潜能；染色体核型为异倍体。结论在体外共培养系统中，成功地建立了1株被胶质瘤干祖细胞SU3诱导恶性转化的永生化树突状细胞株，有望作为工具细胞进一步用于非可控性炎症细胞的相关研究。Objective To establish a malignant transformed cell line by in vitro induction of dendritic cells with glioma stem progenitor cells and to analyze their characteristics. Methods Bone marrow cells were collected from Foxnl^nn B6-CAG-EGFP/SU heterozygous mice expressing enhanced green fluorescent protein （EGFP） by flushing bone marrow cavity with PBS and cultured by using DC culturing method. Then, the cells were co-cultured with glioma stem progenitor cells SU3 expressing red fluorescent protein （RFP） for the con- struction of immortalized cells expressing EGFP. An immortalized cell strain was randomly selected and analyzed by several assays for the identification of DC molecular markers, detection of chromosome karyotype and analysis of tumorigenicity. Results The immortalized cell strain with typical dendritic morphology was named SU3-ih- DCTC （SU3-indnced host dendritic cells transformed tumor cell）. High levels of DC marker proteins （CDllc and CD80） and signal regulated protein alpha （SIRP-α） were detected in the SU3-ihDCTC strain. Moreover, the cell line also expressed host mice evidence marker beta actin （β-aetin） and EGFP. The SU3-ihDCTC strain was a highly proliferative and invasive cell with carcinogenic potential. The chromosome karyotype of the strain was heteroptoid. Conclusion A malignant transformed immortalized dendritic cell line, SU3-ihDCTC, was successfully established by in vitro induction of dendritic cells with glioma stem progenitor cells SU3, which would be useful for further investigation on non-resolving inflammatory cells.

关 键 词：胶质瘤干细胞 恶性转化 绿色荧光蛋白转基因小鼠 肿瘤相关树突状细胞 非可控性炎症细胞 

分 类 号：R739.41[医药卫生—肿瘤]