肝癌患者血清肿瘤标志物含量检测及其与肿瘤组织中JAK-STAT通路的关系研究  

Detection of serum tumor marker contents of liver cancer patients and its correlation with JAK-STAT pathway in tumor tissue

作　　者：董智刚 马丽丽[2] 李亚娟[3] 张占宏[1] 

机构地区：[1]河北省唐山市丰南区医院肝胆外科 [2]河北省唐山市人民医院消化内科,河北唐山063300 [3]河北省唐山市丰南区医院护理部

出　　处：《海南医学院学报》2015年第9期1271-1274,共4页Journal of Hainan Medical University

基　　金：河北省唐山市丰南区医院肝病基金(2014-2015)~~

摘　　要：目的：研究肝癌患者血清肿瘤标志物含量检测及其与肿瘤组织中JAK-STAT通路的关系。方法：选择2013年8月~2014年11月在唐山市丰南区医院确诊为原发性肝癌的50例患者纳入肝癌组,同一时期体检的健康者50例纳入健康组,用荧光定量PCR扩增,检测血清Livin、Xiap、Pim-1、ICAM-1、VCAM-1、CD44v6、DNMT1、DNMT2、DNMT3A、DNMT3B、HDAC1、HDAC5,检测肝脏组织JAK1、JAK2、STAT1、STAT3、STAT5。结果：（1）增殖和黏附分子：与健康组血清中增殖、黏附分子含量比较,肝癌组患者血清中Livin、Xiap、Pim-1、ICAM-1、VCAM-1、CD44v6含量较高（P〈0.05）;（2）甲基转移酶和去乙酰化酶：与健康组血清中甲基转移酶、去乙酰化酶的含量比较,肝癌组血清中DNMT1、DNMT2、DNMT3A、DNMT3B、HDAC1、HDAC5的mRNA含量较高（P〈0.05）;（3）信号分子：与癌旁正常组织中JAK-STAT信号分子含量比较,肝癌组织中信号分子JAK1、JAK2、STAT1、STAT3、STAT5的mRNA含量较高（P〈0.05）。结论：肝癌患者血清中增殖和黏附分子、甲基转移酶和去乙酰化酶的含量异常升高,且与肿瘤组织中JAK-STAT通路的异常激活密切相关。Objective：To study the serum tumor marker contents of liver cancer patients and its correlation with HAKSTAT pathway in tumor tissue.Methods：A total of 50 primary liver cancer patients diagnosed in our hospital from August2013 to November 2014 were enrolled in liver cancer group;50cases of healthy persons received physical examination in our hospital during the same period were enrolled as healthy group.Then serum was collected and Livin,Xiap,Pim-1,ICAM-1,VCAM-1,CD44v6,DNMT1,DNMT2,DNMT3 A,DNMT3B,HDAC1 and HDAC5were detected;liver tissue was collected and JAK1,JAK2,STAT1,STAT3 and STAT5were detected.Results：（1）Proliferation and adhesion molecules：compared with serum proliferation and adhesion molecule contents of healthy group,serum Livin,Xiap,Pim-1,ICAM-1,VCAM-1and CD44v6 contents of liver cancer group were higher;（2）Methyltransferase and histone deacetylase：compared with serum methyltransferase and histone deacetylase contents of healthy group,serum mRNA contents of DNMT1,DNMT2,DNMT3 A,DNMT3B,HDAC1 and HDAC5of liver cancer group were higher;（3）Signal molecules：compared with JAK-STAT signal molecule contents in adjacent normal liver tissue,mRNA contents of JAK1,JAK2,STAT1,STAT3 and STAT5in hepatocellular carcinoma tissue were higher.Conclusion：Contents of serum proliferation and adhesion molecules,methyltransferase and histone deacetylase abnormally increase,which is closely related to the abnormal activation of JAK-STAT pathway in tumor tissue.

关 键 词：肝癌 增殖分子 黏附分子 甲基化 去乙酰化 信号通路 

分 类 号：R735.7[医药卫生—肿瘤]