搭桥法诱导肠炎沙门氏菌鞭毛抗原及鉴定  

Bypass method of induction of salmonella enteritidis flagellum antigen and identification

作　　者：张丹 温应铭 赖志坚 

机构地区：[1]惠东县人民医院检验科,惠东516300 [2]惠东县疾病预防控制中心检验科,惠东516300

出　　处：《北方药学》2015年第9期78-79,62,共3页Journal of North Pharmacy

摘　　要：目的：了解搭桥法对诱导肠炎沙门氏菌鞭毛的临床意义。方法：从2013年4-6月两次食物中毒事件中分离获得7株具有典型生化反应、O-抗原为D群但缺失鞭毛抗原的沙门氏菌。采用搭桥法对7株菌株进行诱导培养,使其鞭毛抗原成功恢复。结果：综合该菌的生物学特性、生化反应及血清学试验得到准确的种属及血清型鉴定。结论：该菌鞭毛抗原的成功诱导为肠炎沙门氏菌的血清型鉴定提供了参考,为基层实验室开展沙门氏菌的血清学检验鉴定提供省时、简单、高效的方法,具有积极意义。Object：To study the clinical signifiCance of the bypass method for induction of salmonella enteritidis flagella. Methods：From April to June 2013 food poisoning, 7 strains were isolated by two typical biochemical reaction, O antigen in D group but lack flagella antigen of salmonella. The bypass method is adopted to induction training of 7 strains, make its flagellum antigen successfully restored.Results：The integrated biological characteristics of the bacteria, biochemical and serological test to get accurate and serotype identification between species. Conclusion：The success of the bacterial flagellum antigen induced for salmonella enteritidis, provides the reference of serotype identification for the laboratory to carry out the serological test identification of salmonella provide time saving, simple and efficient method, has positive significance.

关 键 词：搭桥法 肠炎沙门氏菌 鞭毛抗原诱导 鉴定 

分 类 号：R446.5[医药卫生—诊断学]