BlimplmRNA在类风湿关节炎患者外周血单个核细胞的表达及IL-21对其调节作用的研究  

Expression of Blimpl mRNA in the PBMCs of patients with RA and the corresponding regulation of IL-21

作　　者：刘俊文[1] 刘红春[1] 苏利沙[1] 邓少丽[2] 张根豪[1] 张俊华[1] 魏高辉 

机构地区：[1]郑州大学第一附属医院检验科,450052 [2]第三军医大学大坪医院检验科

出　　处：《中华检验医学杂志》2015年第8期552-556,共5页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金（81101199）;河南省科技厅基础研究计划（142300410030）;郑州市科技攻关项目（141PPTGG437）;河南省卫计委医学科技攻关重点项目（201402008）

摘　　要：目的研究IL-21、BlimplmRNA在类风湿关节炎（RA）患者体内的表达及IL-21刺激后对体外培养RA患者外周血单个核细胞（PBMCs）Blimpl表达的影响，探讨IL-21、Blimpl参与RA发病的具体作用机制。方法病例对照研究。收集2012年10月至2013年3月郑州大学第一附属医院住院确诊的RA患者50例和同期健康体检者50名为对照组的外周静脉血，分离血浆及PBMC，ELISA检测血浆IL．21水平，同时检测患者临床指标DAS28和抗环瓜氨酸肽抗体（抗CCP）抗体将其与患者IL一2l水平进行相关性分析。实时荧光定量PCR（qPCR）检测患者PBMCBlimplmRNA表达量；分离RA患者PBMCs进行体外培养，经IL-21和CD40L刺激细胞72h后，检测患者PBMCBlimplmRNA表达量，流式细胞术检测各组细胞CD20阳性B细胞和CDl38阳性细胞所占比例。采用均值t检验、Wilcoxon秩和检验和方差分析等方法进行统计学分析。结果RA患者血清IL-21水平（130．51±11．35）ng／L明显高于健康对照组（25．46±6．05）ng／L，t=5．39，P=0．007，且IL-21水平与RA患者DAS289（r=0．658）和抗CCP抗体（r=0．674）具有相关性（P分别为0．019、0．016）。RA患者PBMCsBlimplmRNA表达水平（1．321±0．110）高于健康对照组（1．000±0．000），Z=-2．48，P〈0．05。IL-21及CIMOL体外刺激后，IL-21组和CD40L＋IL-21组BlimplmRNA表达量分别为（1．084±0．029）、（1．157±0．028），高于对照组（1．000±0．000），P分别为0．002、0．001，CD40L＋IL-21组BlimplmRNA表达水平高于IL．21组（t=4．862，P=0．02）；CD40L组、IL-21组及IL-21＋CD40L组较阴性对照组CD20阳性B细胞比例增加[2．42±0．35、2．63±0．33、6．35（4．85，6．57），F=278．363，P〈0．001]，CDl38阳性细胞所占比例也增加（0．4744-0．110、0．668±0．120、0．955±0．170，F=49．01，P〈0．001），差异均具有统计学意义。结论IL-21可促进RA患者外周单个核细胞BlimplmRNA的�Objective To Investigate the expression of IL-21 and Blimpl mRNA in Rheumatoid arthritis （RA） patients and the influence on the expression of Blimpl in peripheral blood mononuelear cells （PBMCs） of RA patients after IL-21 stimulated; To further explore the mechanism of IL-21 and blimpl in the pathogenesis of RA. Methods Case control study. The samples of peripheral venous blood from 50 RA patients of department of rheumatology of The First Affiliated Hospital of Zhengzhou University and 50 healthy people were collected respectively, then the plasma and PBMCs was separated. IL-21 in plasma was measured by ELISA; the correlation between patients clinical index DAS28, anti-CCP antibody and IL-21 was analyzed. Blimpl mRNA of patients＇ PBMCs was detected by qPCR; PMBCs were isolated from RA patient and then cultured in vitro. Blimpl mRNA level was measured by qPCR and the ratio of CD20 positive B cell and the ratio of CD138 positive cells in all groups were detected by flow cytometry after 72 h stimulated by IL-21 and CD40L Results IL-21 content in RA patient blood plasma （ 130. 51 ± 11.35） ng/L was significantly higher than that in healthy control（25.46 ± 6. 05 ） ng/L, t = 5.39 ,P 〈 0. 05. Besides, IL- 21 level also had a close relativity with patients DAS28 （r = 0. 658 ） and anti-CCP antibody （r = 0. 674, P = 0. 039 and 0. 035）. In addition, the expression level of Blimpl mRNA in RA patient PMBCs （1. 321 ± 0. 11 ） was higher than that in healthy control group （ 1. 000 ± 0. 000）, Z = - 2. 48, P 〈 0. 05. While after IL-21 and/or CD40L stimulation, Blimpl mRNA of IL-21 group and CD40L ＋ IL-21 group（ 1. 084 ±0. 029, 1. 157 ± 0. 028 ） were higher than those of control （ 1. 000 ± 0. 000 ） （ P = 0. 002, P = 0. 001 ）, moreover the expressive level of BlimplmRNA of CIMOL ＋ IL-21 group was higher than that of control group （t = 4. 862, P = 0. 02）. Compared to negative control group, the ratio of CD20 positive B cells [ 2.42 ± 0. 35, 2. 63 ± 0. 33 , 6. 35（4.

关 键 词：关节炎 类风湿 白细胞介素类 阻遏蛋白质类 白细胞 单核 CIMO配体 

分 类 号：R593.22[医药卫生—内科学] R446[医药卫生—临床医学]