铜胁迫对海菜花幼苗生理特征的影响  

Physiological responses of Ottelia acuminatas seedlings to exogenous copper stress

作　　者：朱健[1] 张志红[1] 范菲菲 赵丽华[1] 

机构地区：[1]贵州大学资源与环境工程学院,贵州贵阳550025 [2]贵州省土壤肥料研究所,贵州贵阳550006

出　　处：《江苏农业学报》2015年第4期883-886,共4页Jiangsu Journal of Agricultural Sciences

基　　金：贵州省科学技术基金项目[黔科合J字(2012)2104;黔科合J字LKN(2013)27]

摘　　要：本研究采用营养液培养法,研究了Cu2+胁迫对海菜花幼苗生理生化指标的影响。结果表明,0.01mg/L、0.05 mg/L、0.10 mg/L、0.20 mg/L和0.40 mg/L浓度的Cu2+胁迫处理显著降低海菜花幼苗叶片中叶绿素a、叶绿素b、总叶绿素及类胡萝卜素含量。随着Cu2+浓度的增加,超氧化歧化酶(SOD)、过氧化物酶(POD)活性、可溶性蛋白质、叶片中Cu2+含量先升高后降低;过氧化氢酶(CAT)呈持续降低趋势,且与对照差异显著。Effects of Cu2+ on physiological and biochemical indices of Ottelia acuminatas were studied by means of nutrient solution culture. The exogenous Cu2+ with varieties of concentrations (0. 01 mg/L, 0. 05 mg/L, 0. 10 mg/L, 0. 20 mg/L and 0. 40 mg/L) exhibited inhibitory effects on chlorophyll contents and carotinoid content in O. acuminatas leaves. The activities of superoxide dismutase(SOD),peroxidase(POD), soluble protein and leaf Cu2+ contents showed an "in-crease-first-and-deerease-afterwards" response to the increase of exogenous Cu2+concentration, however, the activity of cat-alase( CAT) was continuously decreasing, indicative of intolerance of O. acuminatas to copper stress.

关 键 词：铜 海菜花 叶绿素 抗氧化酶系统 可溶性蛋白质 

分 类 号：S634.3[农业科学—蔬菜学]