贵州地方猪种及三元商品猪氟烷基因的检测  被引量:11

Detection of the RYR1 Gene in Guizhou Indigenous Pigs and Three-crossbred Pigs

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作  者:冯文武[1] 龙威海 丁玫[2] 陈祥[1,3] 陈村年 

机构地区:[1]贵州大学动物科学学院高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025 [2]贵州省畜牧兽医学校,贵阳550018 [3]贵州省生猪健康养殖工程技术研究中心,贵阳550025

出  处:《生物技术通报》2015年第8期66-70,共5页Biotechnology Bulletin

基  金:贵州省农业动植物育种专项项目[黔农育专字(2011)019号];贵州省农业攻关项目[黔科合NY(2013)3071号];贵州省工程中心建设项目[黔科合农C字(2011)4022号]

摘  要:旨在对贵州地方猪种"抗应激敏感基因专门化品系"的培育提供技术支撑,运用PCR-RFLP技术对白洗猪、黔北黑猪、宗地花猪、糯谷猪和从江香猪5个地方猪种及三元商品猪共151份样本进行了氟烷基因检测,筛选出猪群中应激敏感基因隐性携带型HalNn、抗应激敏感基因型HalNN和应激敏感基因型Halnn。结果表明,贵州5个地方猪种全为HalNN型;三元商品猪群无Halnn型,HalNn型检出率为52.63%,Haln型等位基因频率为26.32%。To provide the technical support for the cultivation of "strain of specialization with anti-stress and sensitive gene" of Guizhou local pig genes, the RYR1 gene in 151 samples from 5 local pig species of Baixi, Qianbei Black, Zongdi Hua, Nuogu, Congjiang Xiang and three- crossbred commercial pig were detected by PCR-RFLP technique. The pigs with HalNn of recessive anti-stress and sensitive genotype, HalNN of anti-stress and sensitive genotype and Halnn of stress and sensitive genotype were screened out. The results showed that all of 5 Guizhou indigenous pigs belonged to HalNN, there was no Halnn gene in the three-crossbred pigs, HalNn accounted for 52.63 % of the samples, and Haln allele frequency was 26.32%.

关 键 词:氟烷基因 RYR1基因检测 繁殖力 PSE肉 肉质 

分 类 号:S828[农业科学—畜牧学]

 

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