胆盐输出泵在胆管癌大鼠胆管组织中的表达状况  

Bile salt export pump expression in bile duct tissues of rats with bile duct cancer

作　　者：张孟瑜[1] 王洁萍[2] 冯春红[1] 李波[1] 夏先明[1] 

机构地区：[1]泸州医学院附属医院肝胆外科,四川省泸州市646000 [2]泸州医学院附属医院康复科,四川省泸州市646000

出　　处：《世界华人消化杂志》2015年第20期3254-3258,共5页World Chinese Journal of Digestology

基　　金：四川泸州医学院附属医院课题基金资助项目;No.12284~~

摘　　要：目的:本研究通过建立胆管癌大鼠模型,以胆盐输出泵(bile salt export pump,Bsep)为研究对象,初步探讨Bsep在胆管癌大鼠中的表达状况,以期为治疗胆管癌的找到新的手段和方法.方法:取♂Wistar大鼠(体质量160 g±8 g)60只,随机分为2组,每组30只:普通饮食对照组(简称对照组)予以普通饮食、3'-甲基-4,4'-二甲基氨偶氮苯(3'-Me-DAB)饮食实验组(简称实验组)予以含3'-Me-DAB饮食.经过20 wk,建立胆管癌大鼠模型,取对照组和实验组胆管组织:(1)石蜡切片后用免疫组织化学SP(streptavidin-perosidase)法检测两组模型胆管组织的Bsep表达的强度;(2)Western blot法检测两组模型胆管组织的Bsep蛋白表达的强度.结果:免疫组织化学SP法及Western blot法结果均显示,对照组Bsep表达阳性率高于实验组(66.21%vs 18.75%,X2=10.11,P<0.05;0.886±0.017 vs 0.297±0.011,P<0.05).结论:实验组大鼠的Bsep基因的表达较对照组减少,提示我们在胆管癌的治疗中,Bsep有成为新的治疗靶点的可能.AIM： To develop a rat model of bile duct cancer and detect bile salt export pump （Bsep） expre- ssion in bile duct tissues of this model, in order to provide a new method for the preventionand treatment of bile duct cancer METHODS： Sixty Wistar rats were randomly divided into either a control group or an experimental group, with 30 rats in each group. The control group was fed an ordinary diet, and the experimental group was fed a 3＇-Me-DAB diet. After 20 wk, the bile duct cancer model was successfully established. Bile duct tissues were taken from rats in both groups to detect the expression of Bsep by immunohistochemistry （streptavidin-peroxidase） and Western blot. RESULTS： Both immunohistochemistry and Western blot analyses showed that the expres- sion levels of Bsep were significantly higher in the control group that in the experiment group （66.21% vs 18.75%, 2 = 10.11, P 〈 0.05; 0.886 ± 0.017 vs 0.297 ± 0.011, P 〈 0.05）. CONCLUSION： The expression of Bsep protein decreases significantly in rats with bile duct cancer, which suggests that drugs targeting Bsep may be a new therapeutic strategy for bile duct cancer.

关 键 词：胆盐输出泵基因 胆管癌 

分 类 号：R735.8[医药卫生—肿瘤]