双环醇治疗大鼠慢性酒精性肝病的机制探讨  

Exploration on mechanism of bicyclol in the treatment of rats with chronic ALD

作　　者：董力[1] 王建[2] 贾晨虹[3] 郭琳娜[4] 

机构地区：[1]河北省廊坊市人民医院药学部,河北廊坊065000 [2]中国石油天然气总公司中心医院药剂科,河北廊坊065000 [3]河北省廊坊市中医医院药房,河北廊坊065000 [4]河北省廊坊市人民医院特需病房,河北廊坊065000

出　　处：《实用临床医药杂志》2015年第15期1-4,共4页Journal of Clinical Medicine in Practice

基　　金：中国高校医学期刊临床专项资金(11522940)

摘　　要：目的探讨双环醇治疗大鼠慢性酒精性肝病(ALD)的可能机制。方法 72只SD大鼠随机分为对照组、模型组和干预组,各24只。模型组和干预组分别给予白酒灌胃造模,干预组于第4周开始每日加用双环醇灌胃,200 mg/(kg·d),连续给药4周;对照组给予等体积的生理盐水。结果模型组肝脏呈进行性肝细胞损伤坏死及脂肪变性,干预组给药后病变程度显著轻于模型组大鼠。模型组第6、8周肝脏谷胱甘肽(GSH)含量及乙醛脱氢酶(ALDH)活性低于对照组(P<0.05或P<0.01)。干预组第6周GSH含量低于对照组,高于模型组(P<0.05),第8周GSH含量及乙醇脱氢酶(ADH)、ALDH活性均高于模型组(P<0.05)。第6周,模型组大鼠血清谷丙转氨酶(ALT)、胆固醇(TG)、甘油三酯(TC)、低密度脂蛋白胆固醇(LDLC)含量高于对照组(P<0.05或P<0.01);干预组大鼠TG低于模型组(P<0.01)。第8周,模型组大鼠血清各指标与对照组差异显著(P<0.05);干预组上述各指标与模型组差异均有统计学意义(P<0.05或P<0.01)。结论双环醇能有效抑制ALD导致的肝脏脂肪堆积,对酒精引起的肝组织、肝脏细胞结构损伤及肝功能损伤具有良好的保护作用。Objective To explore the potential mechanism of bieyelol in the treatment of rats with chronic alcoholic liver disease （ALD）. Methods A total of 72 SD rats with chronic ALD were randomly divided into control group, model group and intervention group, 24 cases for each group. Model group and intervention group were gavaged with white wine to establish ALD models, after which intervention group was garaged with bicyclol 200 mg/（kg＂ d） from the fourth week, for continuously 4 weeks, while control group was given isometric normal saline. Results Livers in model group showed aggressive hepatoeyte injury, necrosis and fatty degeneration, and the severity of liver lesion was less in intervention group after treatment than that in model group. The liver glu- tathione （GSH） content and acetaldehyde dehydrogenase （ALDH） activity were evidently lower in model group after 6 and 8 weeks than in control group （P〈0.05 or P〈0.01）. Intervention group was markedly lower than control group but higher than model group in GSH content after 6-week treatment （P 〈 0.05）, and was obviously higher in GSH content and alcohol dehydrogenase （ADH） andALDH activities than model group after 8 - week treatment （ P 〈 0 . 0 5 ） . 6 weeks after treatment, model group was markedly higher than control group in serum alanine transaminase （ALT）, cholesterol （TG）, triglyceride （TC） and low-density lipoprotein cholesterol （LDL-C） levels （P 〈 0.05 or P 〈 0.01 ）, and intervention group was apparently lower than model group in TG level （P〈 0.01）. 8 weeks after treatment, there were significant differences between model group and control group in the levels of all serum indexes （P 〈 0.05）, so did the differences between model group and intervention group （P〈0.05 or P〈0.01）. Conclusion Bicyclol can ef- fectively inhibit the accumulation of ALD-induced liver fat and has favorable protective function on alcohol-induced injury of hepatic tissues, hepatocyte structure and hepati

关 键 词：双环醇 慢性酒精性肝病 肝细胞 脂肪变性 

分 类 号：R575[医药卫生—消化系统]