p16基因甲基化在胃癌发病机制中的作用  

Role of p16 gene methylation in the pathogenesis of gastric cancer

作　　者：崔勇[1] 张刚[1] 李中[1] 林晓萌[1] 张军华[1] 

机构地区：[1]河北大学附属医院肿瘤外科,河北保定071000

出　　处：《实用临床医药杂志》2015年第15期49-51,共3页Journal of Clinical Medicine in Practice

基　　金：中国高校医学期刊临床专项资金(11521302)

摘　　要：目的通过检测胃癌组织p16基因的启动子Cp G岛甲基化状态,探讨其在胃癌发病机制中的作用,及其与胃癌临床病理特征之间的关系。方法选取经胃镜检查诊断为胃癌的74例患者(胃癌组)及36例胃黏膜组织正常者(对照组),应用甲基化特异性PCR(MSP)检测正常胃黏膜组织和胃癌组织p16基因的启动子Cp G岛的甲基化状态,并分析p16基因甲基化与胃癌临床病理特征之间的关系。结果胃癌组p16基因的甲基化阳性率为56.8%,对照组未发现p16基因甲基化,差异具有统计学意义(P<0.05);p16基因甲基化表达与胃癌患者的淋巴结转移、远处转移及病变的浸润深度有关(P<0.05),但与性别、年龄、肿瘤大小、部位和组织学类型无关。结论在胃癌发生发展中,p16基因甲基化修饰起着非常重要的作用,可作为反映胃癌侵袭转移的生物学指标。Objective To explore the effect of p16 gene methylation in the pathogenesis of gastric cancer by detecting the methylation state of promoter CpG island of p16 gene in gastric cancer tissue, and its relationship with the clinicopathological features. Methods The methylation state of promoter CpG island of p16 gene in tissues of 74 patients with gastric cancer （gastric cancer group） and 36 normal people （control group） confirmed by stomachoscopy was detected by methylation specif- ic PCR （MSP） for analyzing the relationship between the p16 gene methylation and clinicopathological features. Results The positive methylation rate of p16 gene in gastric cancer group was 56.8%, with significant difference compared with control group （P 〈 0.05 ）, for p16 gene methylation was not found in control group. Moreover, the expression of p16 gene methylation was related to lymphatic metasta- sis, distant metastasis, depth of infiltration of lesion （ P 〈 0.05 ）, but not associated with gender, age, tumor size, lesion location and histological type （ P 〉 0.05 ）. Conclusion p 16 gene methylation modification, which plays an important role in pathogenesis of gastric cancer, can be a biological indicator of reflexing invasion and metastasis of gastric.

关 键 词：P16基因 甲基化 胃癌 生物学指标 

分 类 号：R735.2[医药卫生—肿瘤]