高效液相色谱法测定维生素E软胶囊中的9种对羟基苯甲酸酯类防腐剂  

Determination of 9 parabens preservatives in vitamin E soft capsules by HPCL

作　　者：陈琪琪 王惠娟 茅力[1] 

机构地区：[1]现代毒理学教育部重点实验室南京医科大学公共卫生学院卫生检验学系,江苏南京211166

出　　处：《中国卫生检验杂志》2015年第14期2257-2260,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB911003);国家自然科学基金项目(21275069)

摘　　要：目的建立高效液相色谱（HPLC）法测定维生素E软胶囊中9种对羟基苯甲酸酯类防腐剂。方法分别在酸、碱条件下对软胶囊样品进行萃取-反萃取前处理。采用Aglient Extend-C18（250 mm×4.6 mm,5μm）色谱柱,柱温35℃;以甲醇-10 mmol/L高氯酸为流动相,流速为1.0 ml/min;检测波长为254 nm,外标法定量。结果 9种对羟基苯甲酸酯在选定的色谱条件下分离良好,且分别在不同的浓度范围内与峰面积呈良好的线性关系,相关系数均〉0.999 0。方法的平均回收率为78.6%~111.0%,相对标准偏差为5.4%~13.9%。结论该方法克服了维生素E软胶囊中其他脂溶性成分对测定的影响,且操作简单、结果准确、专属性强,可用于测定维生素E软胶囊中的对羟基苯甲酸酯类防腐剂的含量。Objective To establish a method for determination of 9 parabens preservatives in vitamin E soft capsules by high performance liquid chromatography（ HPLC）. Methods The parabens samples from vitamin E soft capsules were pre- treated for extraction and back- extraction respectively at acidic and basic conditions. The detection was performed on Aglient Extend-C18（ 250 mm × 4. 6 mm,5 μm） column at the column temperature of 35 ℃. The mobile phase consisted of methanol-10 mmol / L perchloric acid at a flow rate of 1. 0 ml / min. The detection wavelength was 254 nm. The quantitative determination was performed by using external standard method. Results The 9 parabens were separated well under the proposed chromatographic condition. And good linearities were obtained from the respective concentration ranges and the peak area with the correlation coefficients more than 0. 999 0. The average recoveries rate ranged from 78. 6% to 110. 0% with the relative standard deviations of 5. 4%- 13. 9%. Conclusion The interference of other fat- soluble substances on the determination of parabens was avoided by this method. This method is simple,accurate,specific,and can be used for simultaneous determination of parabens in vitamin E soft capsules.

关 键 词：高效液相色谱法 维生素E软胶囊 对羟基苯甲酸酯类化合物 防腐剂 

分 类 号：O657.72[理学—分析化学]