蒸发光散射检测器液相色谱法检测酒类饮料中的5种生物胺  

Determination of five biogenic amines alcoholic drinks by LC with evaporative light scattering detector

作　　者：张文豪[1] 张伟[1] 张洁[1] 银恭举[1] 张丁[1] 

机构地区：[1]河南省疾病预防控制中心卫生检测检验中心,河南郑州450016

出　　处：《中国卫生检验杂志》2015年第14期2304-2306,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立一种样品无需衍生化反应,简单快速检测酿制型酒类饮料中5种生物胺的液相色谱分析方法。方法采用蒸发光散射检测器,反相高效液相色谱检测酒类中的5种生物胺。结果在1.0 mg/L^50.0 mg/L时,液相色谱的蒸发光散射检测器的响应值与酒类中5种生物胺含量的三次方相关,5种生物胺的检出限为0.16 mg/L^0.97 mg/L,测定的相对标准偏差<5%,隔天精密度为1.8%~6.7%,加标回收率为90.5%~103.8%。。结论该方法能在25 min内简单快速测定红酒、啤酒和葡萄酒中的组胺、尸胺、腐胺、酪胺、色胺和精胺的含量,具有无需衍生化反应、重现性好、定量准确等优点。Objective To establish a new,simple and rapid high performance liquid chromatography method（ HPLC） to determine five biogenic amines in alcoholic drinks without derivatisation reaction. Methods Five biogenic amines in alcoholic drinks were determined by the reverse HPCL with an evaporative light scattering detector. Results At 1. 0 mg / L- 50. 0 mg / L,the response value of the liquid chromatography evaporative light scattering was correlated with the three times of content of the5 biogenic amines in alcoholic liquor,the detection limit of them was among 0. 16 mg / L- 0. 97 mg / L,the relative standard deviation（ RSD） was less than 5%,and the intermediate precision ranged from 1. 8% to 6. 7%. The recovery varied from 90. 5%to 103. 8%. Conclusion In 25 min the method is able to accurately determine the content of histamine,cadaverine,putrescine,tyramine,tryptamine and sperminein alcoholic drinks with a lot of advantages,such as good reproduction,accurate quantization and without derivatisation reaction.

关 键 词：生物胺 高效液相色谱法 酒类饮料 蒸发光散射 

分 类 号：O657.72[理学—分析化学]