High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges  

作　　者：Dong-Sheng Xue Jiang-Bo Wang Shan-Jing Yao 

机构地区：[1]Ministry of Education of Fermentation Key Laboratory and Collaboration Innovation Centre of Industrial Fermentation, Hubei University of Technology [2]Department of Chemical and Biological Engineering, Zhejiang University

出　　处：《Chinese Chemical Letters》2015年第8期1011-1015,共5页中国化学快报（英文版）

摘　　要：To produce β-glucosidase by consecutive batch fermentation, a marine Aspergillus niger was immobilized on a natural carrier, towel gourd vegetable sponges. The immobilized mycelia were 0.15 g/g carrier with the immobilized biomass percentage of over 95%. The immobilized mycelia possessed the long durability（22.5 days）. The maximum production occurred 1.5 day earlier by the immobilized mycelia than by the free mycelia. β-Glucosidase production of five consecutive batches was over 110 U/m L. At high salinity,the activity and stability of β-glucosidase from the marine A. niger increased remarkable. Immobilizing the marine A. niger on the novel natural carrier achieved the efficient production of β-glucosidase.To produce β-glucosidase by consecutive batch fermentation, a marine Aspergillus niger was immobilized on a natural carrier, towel gourd vegetable sponges. The immobilized mycelia were 0.15 g/g carrier with the immobilized biomass percentage of over 95%. The immobilized mycelia possessed the long durability（22.5 days）. The maximum production occurred 1.5 day earlier by the immobilized mycelia than by the free mycelia. β-Glucosidase production of five consecutive batches was over 110 U/m L. At high salinity,the activity and stability of β-glucosidase from the marine A. niger increased remarkable. Immobilizing the marine A. niger on the novel natural carrier achieved the efficient production of β-glucosidase.

关 键 词：glucosidase niger vegetable batch durability earlier salinity biomass reserved producing 

分 类 号：TQ925[轻工技术与工程—发酵工程] P732.6[天文地球—海洋科学]