不同生长年限黄连不同部位中酚酸类成分动态的研究  

Study on Dynamic State of Phenolic Acids Contents in Different Parts and Growth Years of Coptis Chinensis

作　　者：张丹[1,2] 钟国跃[3] 瞿显友[4,5] 曹纬国[2] 廖光平[4,5] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]重庆医科大学中医药学院,重庆400016 [3]江西中医药大学中药资源与民族药研究中心,南昌330004 [4]重庆市中药资源学重点实验室,重庆400065 [5]中国中医科学院中药资源中心重庆分中心,重庆400065

出　　处：《世界科学技术-中医药现代化》2015年第5期1025-1030,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：中医药管理局国家公益性行业科研专项课题(201207002-04):重庆市代表性区域特色中药资源保护与利用;负责人:钟国跃;重庆市科委科技平台与基地建设项目(cstc2012pt-sy0006):中药资源学重庆重点实验室;负责人:瞿显友

摘　　要：目的:建立黄连不同部位中酚酸类成分的分析方法,探讨不同生长年限黄连不同部位中酚酸类成分的动态变化。方法:采用Fe Cl3-K3[Fe(CN)6]比色法和HPLC法分别测定不同生长年限黄连不同部位中总酚酸、绿原酸与阿魏酸的含量。结果:测得黄连不同部位总酚酸含量约98.435-184.456 mg·g-1在范围内,绿原酸与阿魏酸含量分别在0.176-2.227 mg·g-1与0.039-0.512 mg·g-1范围内。结论:黄连不同部位酚酸类成分含量差异较大,各部位酚酸类成分的含量均表现为移栽2年后最高,进而随生长年限的增加总体呈现下降趋势。This study was aimed to establish a method to determine the content of phenolic acids in different parts of Coptis chinensis, in order to discuss the dynamic change of phenolic acids contents in different parts and growth years of Coptis chinensis. Contents of total phenolic acid, chlorogenic acid and ferulic acid were determined by ferric chloride-ferricyanatum calcium colorimetric method and HPLC, respectively. The results showed that the content of total phenolic acid in Coptis chinensis was in the range from 98.435 mg·g^-1 to 184.456 mg·g^-1. The content of chlorogenic acid and ferulic acid was in the range from 0.176 mg·g^-1 to 2.227 mg·g^-1, and 0.039 mg·g^-1 to 0.512 mg·g^-1, respectively. It was concluded that the content of phenolic acids in different parts of Coptis chinensis were significantly different. The phenolic acids contents in different parts of Coptis chinensis reached the highest two years after transplantation, and then it expressed downswing with the increasing of growth period.

关 键 词：黄连 不同生长年限 不同部位 总酚酸 绿原酸 阿魏酸 

分 类 号：R284[医药卫生—中药学]